PMID- 24462582
OWN - NLM
STAT- MEDLINE
DCOM- 20141031
LR  - 20140224
IS  - 1872-9754 (Electronic)
IS  - 0197-0186 (Linking)
VI  - 66
DP  - 2014 Jan
TI  - The expression of neurotrophins is differentially regulated by omega-3 
      polyunsaturated fatty acids at weaning and postweaning in C57BL/6 mice cerebral 
      cortex.
PG  - 33-42
LID - S0197-0186(14)00008-4 [pii]
LID - 10.1016/j.neuint.2014.01.007 [doi]
AB  - Omega-3 (n-3) polyunsaturated fatty acids (PUFA) and neurotrophins are pivotal to 
      the proper functioning of the central nervous system. We investigated the effects 
      of perinatal and postweaning n-3 PUFA diets on cerebral cortical phospholipid 
      fatty acid composition, and the expression of neurotrophins at weaning and 16 
      weeks postweaning of the male offspring of C57BL/6 mice. Female C57BL/6 mice were 
      fed semi-purified diets (20%w/w fat) containing 10% (high) and 2% (low) n-3 PUFA 
      before mating, during pregnancy, and until weaning. Offspring were studied at 
      weaning and 16 weeks postweaning on their mother's designated diet. Cerebral 
      cortical phospholipid fatty acids and mRNA expressions of brain-derived 
      neurotrophic factor (BDNF), nerve growth factor (NGF), cAMP response element 
      binding protein (CREB), and TrkB were measured. The protein concentration of 
      phosphorylated CREB (pCREB) was determined by ELISA. DHA and total n-3 PUFA were 
      significantly higher in cortical phospholipids of the high n-3 PUFA group 
      compared to the low n-3 PUFA group (P<0.0001), and increased significantly from 
      weaning to 16 weeks (P<0.0001). There was a significant effect of diet (P<0.05) 
      and age (P<0.05) on the mRNA expression of NGF. The mRNA expression of BDNF 
      increased significantly (P<0.01) in the high n-3 PUFA group compared to the low 
      n-3 PUFA group. The mRNA expression of TrKB was significantly higher (P<0.0001) 
      at 16 weeks in the high n-3 PUFA group compared to the low n-3 PUFA group; 
      however, no difference was observed at weaning. pCREB/Total CREB was higher in 
      the high n-3 PUFA group compared to the low n-3 PUFA group (P<0.05). Our findings 
      demonstrate that perinatal and postweaning diets high in n-3 PUFA lead to 
      accretion of n-3 PUFA in brain cortex. We further found that n-3 PUFA upregulates 
      the expression of neurotrophins and their target receptors in an age dependent 
      fashion.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Balogun, Kayode A
AU  - Balogun KA
AD  - Department of Biochemistry, Memorial University, St. John's, NL, Canada.
FAU - Cheema, Sukhinder K
AU  - Cheema SK
AD  - Department of Biochemistry, Memorial University, St. John's, NL, Canada. 
      Electronic address: skaur@mun.ca.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140122
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Fatty Acids, Omega-3)
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Cerebral Cortex/drug effects/growth & development/*metabolism
MH  - Fatty Acids, Omega-3/*administration & dosage
MH  - Female
MH  - *Gene Expression Regulation
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Growth Factors/*biosynthesis
MH  - Pregnancy
MH  - *Weaning
OTO - NOTNLM
OT  - Brain development
OT  - Neurotrophins
OT  - Perinatal
OT  - Postweaning
OT  - n-3 PUFA
OT  - pCREB
EDAT- 2014/01/28 06:00
MHDA- 2014/11/02 06:00
CRDT- 2014/01/28 06:00
PHST- 2013/11/12 00:00 [received]
PHST- 2013/12/19 00:00 [revised]
PHST- 2014/01/14 00:00 [accepted]
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2014/11/02 06:00 [medline]
AID - S0197-0186(14)00008-4 [pii]
AID - 10.1016/j.neuint.2014.01.007 [doi]
PST - ppublish
SO  - Neurochem Int. 2014 Jan;66:33-42. doi: 10.1016/j.neuint.2014.01.007. Epub 2014 
      Jan 22.