PMID- 24462687
OWN - NLM
STAT- MEDLINE
DCOM- 20140414
LR  - 20140224
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 588
IP  - 5
DP  - 2014 Mar 3
TI  - Decreased IL-10 expression in stefin B-deficient macrophages is regulated by the 
      MAP kinase and STAT-3 signaling pathways.
PG  - 720-6
LID - S0014-5793(14)00041-6 [pii]
LID - 10.1016/j.febslet.2014.01.015 [doi]
AB  - Innate immune responses are tightly regulated to avoid excessive activation and 
      subsequent inflammatory damage to the host, and interleukin-10 (IL-10) plays a 
      crucial role in preventing inflammation. Stefin B (cystatin B) is an endogenous 
      inhibitor of cysteine proteinases. In stefin B-deficient bone marrow-derived 
      macrophages (BMDMs), we detected an increase in the induction of the LPS-induced 
      pro-inflammatory signal nitric oxide (NO) but decreased IL-10 expression. The 
      phosphorylation of ERK and p38 MAP-kinases was significantly decreased in stefin 
      B-deficient macrophages, as was STAT-3 phosphorylation. These findings show that 
      stefin B influences the expression of anti-inflammatory IL-10 in response to the 
      TLR4 agonist LPS.
CI  - Copyright (c) 2014 Federation of European Biochemical Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Maher, Katarina
AU  - Maher K
AD  - Department of Biochemistry, Molecular and Structural Biology, Jozef Stefan 
      Institute, Ljubljana SI-1000, Slovenia; Jozef Stefan International Postgraduate 
      School, Jamova 39, SI-1000 Ljubljana, Slovenia.
FAU - Zavrsnik, Janja
AU  - Zavrsnik J
AD  - Department of Biochemistry, Molecular and Structural Biology, Jozef Stefan 
      Institute, Ljubljana SI-1000, Slovenia; Jozef Stefan International Postgraduate 
      School, Jamova 39, SI-1000 Ljubljana, Slovenia.
FAU - Jeric-Kokelj, Barbara
AU  - Jeric-Kokelj B
AD  - Department of Biochemistry, Molecular and Structural Biology, Jozef Stefan 
      Institute, Ljubljana SI-1000, Slovenia; Jozef Stefan International Postgraduate 
      School, Jamova 39, SI-1000 Ljubljana, Slovenia.
FAU - Vasiljeva, Olga
AU  - Vasiljeva O
AD  - Department of Biochemistry, Molecular and Structural Biology, Jozef Stefan 
      Institute, Ljubljana SI-1000, Slovenia.
FAU - Turk, Boris
AU  - Turk B
AD  - Department of Biochemistry, Molecular and Structural Biology, Jozef Stefan 
      Institute, Ljubljana SI-1000, Slovenia; Centre of Excellence for Integrated 
      Approaches in Chemistry and Biology of Proteins (CIPKeBiP), Jamova 39, SI-1000 
      Ljubljana, Slovenia; Department of Chemistry and Biochemistry, Faculty of 
      Chemistry and Chemical Technology, University of Ljubljana, Cesta v Mestni log 
      88A, SI-1000 Ljubljana, Slovenia.
FAU - Kopitar-Jerala, Natasa
AU  - Kopitar-Jerala N
AD  - Department of Biochemistry, Molecular and Structural Biology, Jozef Stefan 
      Institute, Ljubljana SI-1000, Slovenia. Electronic address: 
      natasa.kopitar@ijs.si.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140123
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Stat3 protein, mouse)
RN  - 130068-27-8 (Interleukin-10)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 88844-95-5 (Cystatin B)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cystatin B/*deficiency/genetics
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Interferon-gamma/genetics
MH  - Interleukin-10/genetics/*metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - MAP Kinase Signaling System
MH  - Macrophage Activation
MH  - Macrophages/immunology/*metabolism
MH  - Mice, Knockout
MH  - Nitric Oxide/metabolism
MH  - STAT3 Transcription Factor/*metabolism
MH  - Transcriptional Activation/immunology
OTO - NOTNLM
OT  - Cystatin
OT  - Interleukin-10
OT  - Lipopolysaccharide
OT  - MAP-kinase
OT  - Macrophage
OT  - Nitric oxide
EDAT- 2014/01/28 06:00
MHDA- 2014/04/15 06:00
CRDT- 2014/01/28 06:00
PHST- 2013/09/16 00:00 [received]
PHST- 2014/01/07 00:00 [revised]
PHST- 2014/01/08 00:00 [accepted]
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2014/04/15 06:00 [medline]
AID - S0014-5793(14)00041-6 [pii]
AID - 10.1016/j.febslet.2014.01.015 [doi]
PST - ppublish
SO  - FEBS Lett. 2014 Mar 3;588(5):720-6. doi: 10.1016/j.febslet.2014.01.015. Epub 2014 
      Jan 23.