PMID- 24462779
OWN - NLM
STAT- MEDLINE
DCOM- 20150204
LR  - 20140421
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 116
DP  - 2014 Apr 1
TI  - Optimized immobilization of lectins using self-assembled monolayers on 
      polysilicon encoded materials for cell tagging.
PG  - 104-13
LID - S0927-7765(13)00808-4 [pii]
LID - 10.1016/j.colsurfb.2013.12.053 [doi]
AB  - Self-assembled monolayers (SAMs) have been used for the preparation of functional 
      microtools consisting of encoded polysilicon barcodes biofunctionalized with 
      proteins of the lectin family. These hybrid microtools exploit the lectins 
      ability for recognizing specific carbohydrates of the cell membrane to give an 
      efficient system for cell tagging. This work describes how the control of the 
      methodology for SAM formation on polysilicon surfaces followed by lectin 
      immobilization has a crucial influence on the microtool biofunction. Several 
      parameters (silanization time, silane molar concentration, type of solvent or 
      deposition methodology) have been studied to establish optimal function. 
      Furthermore, silanes incorporating different terminal groups, such as aldehyde, 
      activated ester or epoxide groups were tested in order to analyze their chemical 
      coupling with the biomolecules, as well as their influence on the 
      biofunctionality of the immobilized protein. Two different lectins - wheat germ 
      agglutinin (WGA) and phytohemagglutinin (PHA-L) - were immobilized, because they 
      have different and specific cell recognition behaviour and exhibit different cell 
      toxicity. In this way we can assess the effect of intrinsic bulk toxicity with 
      that of the cell compatibility once immobilized as well as the importance of cell 
      affinity. A variety of nanometrical techniques were used to characterize the 
      active surfaces, and lectin immobilization was quantified using 
      ultraviolet-visible absorption spectroscopy (UV-vis) and optical waveguide light 
      mode spectroscopy (OWLS). Once the best protocol was found, WGA and PHA were 
      immobilized on polysilicon coded barcodes, and these microtools showed excellent 
      cell tagging on living mouse embryos when WGA was used.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Penon, Oriol
AU  - Penon O
AD  - Department of Pharmacology and Therapeutical Chemistry and Institute of 
      Nanoscience and Nanotechnology UB (IN2UB), Universitat de Barcelona, Avda. Joan 
      XXIII s/n, 08028 Barcelona, Spain.
FAU - Siapkas, Dimitrios
AU  - Siapkas D
AD  - Department of Pharmacology and Therapeutical Chemistry and Institute of 
      Nanoscience and Nanotechnology UB (IN2UB), Universitat de Barcelona, Avda. Joan 
      XXIII s/n, 08028 Barcelona, Spain.
FAU - Novo, Sergi
AU  - Novo S
AD  - Department of Cellular Biology, Physiology and Immunology, Universitat Autonoma 
      de Barcelona, 08193 Bellaterra, Spain.
FAU - Duran, Sara
AU  - Duran S
AD  - Institute of Microelectronics of Barcelona, IMB-CNM (CSIC), Campus UAB, 08193 
      Bellaterra, Barcelona, Spain.
FAU - Oncins, Gerard
AU  - Oncins G
AD  - Scientific and Technological Centers of the Universitat de Barcelona, Lluis Sole 
      i Sabaris 1, 08028, Barcelona, Spain.
FAU - Errachid, Abdelhamid
AU  - Errachid A
AD  - Universite de Lyon, Lyon1, Institut des Sciences Analytiques (ISA), UMR 5280, 5 
      Rue de la Doua, 69100 Villeurbanne Cedex, France.
FAU - Barrios, Lleonard
AU  - Barrios L
AD  - Department of Cellular Biology, Physiology and Immunology, Universitat Autonoma 
      de Barcelona, 08193 Bellaterra, Spain.
FAU - Nogues, Carme
AU  - Nogues C
AD  - Department of Cellular Biology, Physiology and Immunology, Universitat Autonoma 
      de Barcelona, 08193 Bellaterra, Spain.
FAU - Duch, Marta
AU  - Duch M
AD  - Institute of Microelectronics of Barcelona, IMB-CNM (CSIC), Campus UAB, 08193 
      Bellaterra, Barcelona, Spain.
FAU - Plaza, Jose Antonio
AU  - Plaza JA
AD  - Institute of Microelectronics of Barcelona, IMB-CNM (CSIC), Campus UAB, 08193 
      Bellaterra, Barcelona, Spain.
FAU - Perez-Garcia, Lluisa
AU  - Perez-Garcia L
AD  - Department of Pharmacology and Therapeutical Chemistry and Institute of 
      Nanoscience and Nanotechnology UB (IN2UB), Universitat de Barcelona, Avda. Joan 
      XXIII s/n, 08028 Barcelona, Spain. Electronic address: mlperez@ub.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131230
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Lectins)
RN  - 0 (Polymers)
RN  - Z4152N8IUI (Silicon)
SB  - IM
MH  - Animals
MH  - Cell Adhesion
MH  - Cell Membrane/chemistry
MH  - Lectins/*chemistry
MH  - Mice
MH  - Polymers/chemical synthesis/*chemistry
MH  - Silicon/*chemistry
MH  - Surface Properties
OTO - NOTNLM
OT  - Cell tagging
OT  - Immobilization
OT  - Lectins
OT  - Polysilicon
OT  - Self-assembled monolayers
OT  - Surface chemistry
EDAT- 2014/01/28 06:00
MHDA- 2015/02/05 06:00
CRDT- 2014/01/28 06:00
PHST- 2013/09/02 00:00 [received]
PHST- 2013/12/11 00:00 [revised]
PHST- 2013/12/21 00:00 [accepted]
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2015/02/05 06:00 [medline]
AID - S0927-7765(13)00808-4 [pii]
AID - 10.1016/j.colsurfb.2013.12.053 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2014 Apr 1;116:104-13. doi: 
      10.1016/j.colsurfb.2013.12.053. Epub 2013 Dec 30.