PMID- 24463128
OWN - NLM
STAT- MEDLINE
DCOM- 20150105
LR  - 20200914
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 34
IP  - 1
DP  - 2014 Jan
TI  - [Effects of exercise of different intensity on early repair of full-thickness 
      articular cartilage defects and expressions of MMP-3 and TIMP-1 in rats].
PG  - 103-8
AB  - OBJECTIVE: To evaluate the effects of treadmill running exercise of different 
      intensity on early repair of full-thickness defects on the patellofemoral 
      articular surface and the changes in the expressions of matrix 
      metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) 
      in SD rats. METHODS: Twenty-four male SD rats with full-thickness defects on the 
      patellofemoral articular surface were randomly assigned into sedentary control 
      (SED) group and low-, moderate- and high-intensity running groups (LIR, MIR, and 
      HIR groups, respectively). The running groups were trained on treadmill for 6 
      consecutive weeks. Blood samples were collected to detect serum MMP-3 and TIMP-1 
      levels using ELISA before and after the experiment, and the femoral trochlea were 
      collected to assess tissue repair by gross appearance scoring and O Driscoll 
      histological scoring with Safranine O-Fast Green staining and Toluidine blue 
      staining. RESULTS: In rats in SED group, the defect was filled with hyaline 
      articular cartilage-like tissues, as compared to fibrous tissues in LIR and MIR 
      groups and subchondral bone damage in HIR group. The SED group scored the highest 
      and HIR group the lowest among the 4 groups in gross appearance scoring and O 
      Driscoll histological scoring. No significant differences were found in MMP-3 or 
      TIMP-1 levels among the groups before training (P>0.05), but after 6 weeks of 
      training, serum MMP-3 and TIMP-1 levels differed significantly among the 4 groups 
      (P<0.05), and all the 3 running groups had a significantly higher MMP-3 level 
      than the control group (P<0.05). After the 6-week training, TIMP-1/MMP-3 ratio 
      was significantly higher in SED group than in the 3 running groups, and was the 
      lowest in HIR group. CONCLUSION: Both low- and moderate-intensity exercise failed 
      to promote resurfacing of full-thickness cartilage defects on the patellofemoral 
      articular surface in rats, and high-intensity exercise even induces subchondral 
      bone damage. The expression of MMP-3 and TIMP-1 is related to exercise, and the 
      TIMP-1/MMP-3 ratio reflects the extent of tissue repair.
FAU - Dong, Fu
AU  - Dong F
AD  - Department of Orthopedics and Traumatology, Nanfang Hospital, Southern Medical 
      University, Guangzhou 510515, China. E-mail: df96502@163.com.
FAU - Song, Jinqi
AU  - Song J
FAU - Luo, Jiwei
AU  - Luo J
FAU - Zhao, Peiran
AU  - Zhao P
FAU - Ni, Guoxin
AU  - Ni G
FAU - Yu, Bin
AU  - Yu B
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (TIMP1 protein, rat)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.7 (MMP1 protein, rat)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/*metabolism/*pathology
MH  - Male
MH  - Matrix Metalloproteinase 1/metabolism
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - *Physical Conditioning, Animal
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tissue Inhibitor of Metalloproteinase-1/*metabolism
MH  - Wound Healing
EDAT- 2014/01/28 06:00
MHDA- 2015/01/06 06:00
CRDT- 2014/01/28 06:00
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2015/01/06 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2014 Jan;34(1):103-8.