PMID- 24463407
OWN - NLM
STAT- MEDLINE
DCOM- 20141210
LR  - 20140421
IS  - 1873-2976 (Electronic)
IS  - 0960-8524 (Linking)
VI  - 155
DP  - 2014 Mar
TI  - Rapid quantification of microalgal lipids in aqueous medium by a simple 
      colorimetric method.
PG  - 330-3
LID - S0960-8524(13)01917-2 [pii]
LID - 10.1016/j.biortech.2013.12.077 [doi]
AB  - Identification of novel microalgal strains with high lipid productivity is one of 
      the most important research topics in renewable biofuel research. However, the 
      major bottleneck in the strain screening process is that currently known methods 
      for the estimation of microalgal lipid are laborious and time-consuming. The 
      present study successfully employed sulpho-phospho-vanillin (SPV) colorimetric 
      method for direct quantitative measurement of lipids within liquid microalgal 
      culture. The SPV reacts with lipids to produce a distinct pink color, and its 
      intensity can be quantified using spectrophotometric methods by measuring 
      absorbance at 530nm. This method was employed for a rapid quantification of 
      intracellular lipid contents within Chlorella sp., Monoraphidium sp., Ettlia sp. 
      and Nannochloropsis sp., all of which were found to have lipid contents ranging 
      in between 10% and 30%. Subsequent analysis of the biomass using gas 
      chromatography confirmed that our protocol is highly accurate (R(2)=0.99).
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Mishra, Sanjiv K
AU  - Mishra SK
AD  - Advanced Biomass R&D Center, #2502 Building W1-3, KAIST, 291 Daehak-ro, 373-1 
      Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
FAU - Suh, William I
AU  - Suh WI
AD  - Advanced Biomass R&D Center, #2502 Building W1-3, KAIST, 291 Daehak-ro, 373-1 
      Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
FAU - Farooq, Wasif
AU  - Farooq W
AD  - Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, 373-1 
      Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
FAU - Moon, Myounghoon
AU  - Moon M
AD  - Department of Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, 373-1 
      Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
FAU - Shrivastav, Anupama
AU  - Shrivastav A
AD  - Advanced Biomass R&D Center, #2502 Building W1-3, KAIST, 291 Daehak-ro, 373-1 
      Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
FAU - Park, Min S
AU  - Park MS
AD  - Advanced Biomass R&D Center, #2502 Building W1-3, KAIST, 291 Daehak-ro, 373-1 
      Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea; Bioscience 
      Division, Los Alamos National Laboratory, Los Alamos, NM 87545, United States. 
      Electronic address: minsungpark0@kaist.ac.kr.
FAU - Yang, Ji-Won
AU  - Yang JW
AD  - Advanced Biomass R&D Center, #2502 Building W1-3, KAIST, 291 Daehak-ro, 373-1 
      Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea; Department of 
      Chemical and Biomolecular Engineering, KAIST, 291 Daehak-ro, 373-1 Guseong-dong, 
      Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: 
      jwyang@kaist.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131227
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 0 (Lipids)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Chromatography, Gas
MH  - Colorimetry/*methods
MH  - Lipids/*analysis
MH  - Microalgae/*chemistry
MH  - Molecular Structure
MH  - Species Specificity
MH  - Water
OTO - NOTNLM
OT  - Colorimetric
OT  - Gas chromatography
OT  - Lipid
OT  - Microalgae
OT  - Vanillin
EDAT- 2014/01/28 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/01/28 06:00
PHST- 2013/11/26 00:00 [received]
PHST- 2013/12/14 00:00 [revised]
PHST- 2013/12/19 00:00 [accepted]
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0960-8524(13)01917-2 [pii]
AID - 10.1016/j.biortech.2013.12.077 [doi]
PST - ppublish
SO  - Bioresour Technol. 2014 Mar;155:330-3. doi: 10.1016/j.biortech.2013.12.077. Epub 
      2013 Dec 27.