PMID- 24463969
OWN - NLM
STAT- MEDLINE
DCOM- 20141015
LR  - 20211021
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 80
IP  - 7
DP  - 2014 Apr
TI  - Sustainable growth of Dehalococcoides mccartyi 195 by corrinoid salvaging and 
      remodeling in defined lactate-fermenting consortia.
PG  - 2133-41
LID - 10.1128/AEM.03477-13 [doi]
AB  - Corrinoids are essential cofactors of reductive dehalogenases in Dehalococcoides 
      mccartyi, an important bacterium in bioremediation, yet sequenced D. mccartyi 
      strains do not possess the complete pathway for de novo corrinoid biosynthesis. 
      Pelosinus sp. and Desulfovibrio sp. have been detected in dechlorinating 
      communities enriched from contaminated groundwater without exogenous cobalamin 
      corrinoid. To investigate the corrinoid-related interactions among key members of 
      these communities, we constructed consortia by growing D. mccartyi strain 195 
      (Dhc195) in cobalamin-free, trichloroethene (TCE)- and lactate-amended medium in 
      cocultures with Desulfovibrio vulgaris Hildenborough (DvH) or Pelosinus 
      fermentans R7 (PfR7) and with both in tricultures. Only the triculture exhibited 
      sustainable dechlorination and cell growth when a physiological level of 
      5,6-dimethylbenzimidazole (DMB), the lower ligand of cobalamin, was provided. In 
      the triculture, DvH provided hydrogen while PfR7 provided corrinoids to Dhc195, 
      and the initiation of dechlorination and Dhc195 cell growth was highly dependent 
      on the growth of PfR7. Corrinoid analysis indicated that Dhc195 imported and 
      remodeled the phenolic corrinoids produced by PfR7 into cobalamin in the presence 
      of DMB. Transcriptomic analyses of Dhc195 showed the induction of the 
      CbiZ-dependent corrinoid-remodeling pathway and BtuFCD corrinoid ABC transporter 
      genes during corrinoid salvaging and remodeling. In contrast, another operon 
      annotated to encode a putative iron/cobalamin ABC transporter (DET1174-DET1176) 
      was induced when cobalamin was exogenously provided. Interestingly, a global 
      upregulation of phage-related genes was observed when PfR7 was present. These 
      findings provide insights into both the gene regulation of corrinoid salvaging 
      and remodeling in Dhc195 when it is grown without exogenous cobalamin and 
      microbe-to-microbe interactions in dechlorinating microbial communities.
FAU - Men, Yujie
AU  - Men Y
AD  - Department of Civil and Environmental Engineering, University of California, 
      Berkeley, California, USA.
FAU - Seth, Erica C
AU  - Seth EC
FAU - Yi, Shan
AU  - Yi S
FAU - Allen, Robert H
AU  - Allen RH
FAU - Taga, Michiko E
AU  - Taga ME
FAU - Alvarez-Cohen, Lisa
AU  - Alvarez-Cohen L
LA  - eng
GR  - P42 ES004705/ES/NIEHS NIH HHS/United States
GR  - P42ES004705/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140124
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Benzimidazoles)
RN  - 0 (Corrinoids)
RN  - 0 (Culture Media)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 4R7X1O2820 (Chlorine)
RN  - 553XI1DS20 (5,6-dimethylbenzimidazole)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Benzimidazoles/metabolism
MH  - Chlorine/metabolism
MH  - Chloroflexi/*growth & development/*metabolism
MH  - Corrinoids/*metabolism
MH  - Culture Media/chemistry
MH  - Desulfovibrio vulgaris/growth & development/metabolism
MH  - Fermentation
MH  - Hydrogen/metabolism
MH  - Lactic Acid/*metabolism
MH  - *Microbial Consortia
MH  - Transcriptome
MH  - Veillonellaceae/growth & development/metabolism
PMC - PMC3993147
EDAT- 2014/01/28 06:00
MHDA- 2014/10/16 06:00
PMCR- 2014/10/01
CRDT- 2014/01/28 06:00
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2014/10/16 06:00 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - AEM.03477-13 [pii]
AID - 03477-13 [pii]
AID - 10.1128/AEM.03477-13 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2014 Apr;80(7):2133-41. doi: 10.1128/AEM.03477-13. Epub 
      2014 Jan 24.