PMID- 24464212
OWN - NLM
STAT- MEDLINE
DCOM- 20140826
LR  - 20220409
IS  - 1573-2568 (Electronic)
IS  - 0163-2116 (Linking)
VI  - 59
IP  - 7
DP  - 2014 Jul
TI  - HOXA10 promotes cell invasion and MMP-3 expression via TGFbeta2-mediated activation 
      of the p38 MAPK pathway in pancreatic cancer cells.
PG  - 1442-51
LID - 10.1007/s10620-014-3033-6 [doi]
AB  - BACKGROUND: HOXA10 is closely related to tumor progression in many human cancers. 
      However, the role of HOXA10 in pancreatic cancer remains unclear. The aim of this 
      study was to determine the involvement of HOXA10 in pancreatic cancer cell 
      invasion and migration. METHODS: The effect of HOXA10 on the invasion and 
      migration of pancreatic cancer cells was assessed by invasion and migration 
      assays. The protein of transforming growth factor beta-2 (TGFbeta2) was neutralized 
      by TGFbeta2 blocking antibody. The activation of p38 was inhibited by SB239063. 
      RESULTS: HOXA10 could promote the invasion and migration of pancreatic cancer 
      cells. Knockdown of HOXA10 decreased the expressions of TGFbeta2 and matrix 
      metallopeptidase-3 (MMP-3) and suppressed the activation of p38. Conversely, 
      overexpression of HOXA10 increased the levels of TGFbeta2 and MMP-3. Further 
      experiments identified that TGFbeta2 contributed to the HOXA10-promoted invasion and 
      migration and regulated MMP-3 expression and p38 activation. Additionally, 
      inhibition of p38 suppressed cell invasion and MMP-3 expression in pancreatic 
      cancer cells. CONCLUSIONS: HOXA10 promotes cell invasion and MMP-3 expression of 
      pancreatic cancer cells via TGFbeta2-p38 MAPK pathway. Thus, HOXA10 could be a 
      useful target for the treatment of pancreatic cancer.
FAU - Cui, Xian-Ping
AU  - Cui XP
AD  - Department of Hepatobiliary Surgery, Provincial Hospital Affiliated to Shandong 
      University, 324 Jing Wu Road, Jinan, 250021, China, cuixianp@163.com.
FAU - Qin, Cheng-Kun
AU  - Qin CK
FAU - Zhang, Zhen-Hai
AU  - Zhang ZH
FAU - Su, Zhong-Xue
AU  - Su ZX
FAU - Liu, Xin
AU  - Liu X
FAU - Wang, Shi-Kang
AU  - Wang SK
FAU - Tian, Xing-Song
AU  - Tian XS
LA  - eng
PT  - Journal Article
DEP - 20140125
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Homeobox A10 Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Transforming Growth Factor beta2)
RN  - 140441-81-2 (HOXA10 protein, human)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Biomarkers, Tumor/*metabolism
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Cell Movement/*physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Knockdown Techniques
MH  - Homeobox A10 Proteins
MH  - Homeodomain Proteins/*metabolism
MH  - Humans
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Neoplasm Invasiveness
MH  - Pancreatic Neoplasms/metabolism/pathology/*physiopathology
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transforming Growth Factor beta2/*metabolism
MH  - Up-Regulation
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
EDAT- 2014/01/28 06:00
MHDA- 2014/08/27 06:00
CRDT- 2014/01/28 06:00
PHST- 2013/08/18 00:00 [received]
PHST- 2014/01/08 00:00 [accepted]
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2014/08/27 06:00 [medline]
AID - 10.1007/s10620-014-3033-6 [doi]
PST - ppublish
SO  - Dig Dis Sci. 2014 Jul;59(7):1442-51. doi: 10.1007/s10620-014-3033-6. Epub 2014 
      Jan 25.