PMID- 24464395
OWN - NLM
STAT- MEDLINE
DCOM- 20141230
LR  - 20140425
IS  - 1520-6327 (Electronic)
IS  - 0739-4462 (Linking)
VI  - 86
IP  - 1
DP  - 2014 May
TI  - Suppressing the expression of a forkhead transcription factor disrupts the chitin 
      biosynthesis pathway in Spodoptera exigua.
PG  - 4-18
LID - 10.1002/arch.21145 [doi]
AB  - Forkhead (Fox) transcription factors display functional diversity and are 
      involved in various metabolic and developmental processes. The Spodoptera exigua 
      Fox (SeFox) encodes a protein of 353 amino acids with a theoretical molecular 
      mass of approximately 38.99 kDa and an isoelectric point of 8.86. qPCR results 
      revealed that SeFox was expressed mainly in the brain, fat body, epidermis, 
      midgut, Malpighian tubules, and testis. SeFox was expressed, with some changes, 
      throughout development in the fat body and whole body. Injection of dsSeFox 
      (SeFox dsRNA) into larvae resulted in incidences of albino plus molting deformity 
      (4.8%), molting deformity (26.2%), and albino phenotypes (69.1%). dsSeFox 
      injection resulted in approximately 50% knockdown of transcript levels at 36 h. 
      Compared with control groups, hexokinase (HK) expression was reduced to 
      approximately 40% at 48 h postinjection. Chitin synthase A (CHSA) expression was 
      reduced to two-thirds at 24 h, but increased at 72 h. Compared with untreated 
      control and green fluorescent protein-treated groups, Chitin synthase B (CHSB) 
      expression decreased to 33% following dsSeFox injection by 36 h. We infer from 
      our results that forkhead transcription factors act in chitin synthesis in S. 
      exigua.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Zhao, Lina
AU  - Zhao L
AD  - Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and 
      Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
FAU - Wei, Ping
AU  - Wei P
FAU - Guo, Hongshuang
AU  - Guo H
FAU - Wang, Shigui
AU  - Wang S
FAU - Tang, Bin
AU  - Tang B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140126
PL  - United States
TA  - Arch Insect Biochem Physiol
JT  - Archives of insect biochemistry and physiology
JID - 8501752
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Insect Proteins)
RN  - 1398-61-4 (Chitin)
RN  - EC 2.4.1.16 (Chitin Synthase)
SB  - IM
MH  - Animals
MH  - Chitin/*biosynthesis/genetics
MH  - Chitin Synthase/genetics/metabolism
MH  - Forkhead Transcription Factors/*metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Insect Proteins/genetics/*metabolism
MH  - Larva/metabolism
MH  - Spodoptera/*genetics/growth & development/*metabolism
OTO - NOTNLM
OT  - RNAi
OT  - Spodoptera exigua
OT  - chitin
OT  - chitin synthase
OT  - forkhead
EDAT- 2014/01/28 06:00
MHDA- 2014/12/31 06:00
CRDT- 2014/01/28 06:00
PHST- 2014/01/28 06:00 [entrez]
PHST- 2014/01/28 06:00 [pubmed]
PHST- 2014/12/31 06:00 [medline]
AID - 10.1002/arch.21145 [doi]
PST - ppublish
SO  - Arch Insect Biochem Physiol. 2014 May;86(1):4-18. doi: 10.1002/arch.21145. Epub 
      2014 Jan 26.