PMID- 24466768
OWN - NLM
STAT- MEDLINE
DCOM- 20150108
LR  - 20140128
IS  - 1004-5619 (Print)
IS  - 1004-5619 (Linking)
VI  - 29
IP  - 5
DP  - 2013 Oct
TI  - [Apoptosis in adult mouse brain after chronic poisoning of ketamine].
PG  - 325-9
AB  - OBJECTIVE: To study the effect of chronic poisoning of ketamine on brain cell 
      apoptosis in adult mouse under different duration and doses. METHODS: The mouse 
      model of chronic poisoning of ketamine was established on adult mouse by tail 
      vein injection of ketamine twice every week with different doses (4, 10, 20 and 
      30 mg/kg). The mice were sacrificed after continuous injection of ketamine of 1, 
      2, 4, 8 and 12 weeks. The qualitative assessment of apoptosis was made by 
      transmission electron microscope and the quantitative assessment was made by 
      Caspase-3 immumofluorescence staining method and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) to estimate the time point of 
      apoptosis. All the experimental results were statistically analyzed. RESULTS: The 
      neuron apoptosis was observed in hippocampus and corpus striatum by transmission 
      electron microscope one week after administration, and continued for eight weeks. 
      High level of Caspase-3 expression was observed one week after administration, 
      but with a low level expression after 4 weeks. The number of TUNEL positive cells 
      obviously increased one week after administration and maintained in a high number 
      at 4 weeks. CONCLUSION: Ketamine by tail vein injection could induce neuron 
      apoptosis in adult mouse.
FAU - Yang, Ju
AU  - Yang J
AD  - Department of Pathology, the Fifth People's Hospital of Shanghai, Fudan 
      University, Shanghai 200240, China. yangju_33@126.com
FAU - Li, Xiao-jing
AU  - Li XJ
AD  - Department of Pathology, the Fifth People's Hospital of Shanghai, Fudan 
      University, Shanghai 200240, China.
FAU - Zhang, Zhi-xiang
AU  - Zhang ZX
AD  - Department of Forensic Medicine, Medical College of Soochow University, Suzhou 
      215123, China.
FAU - Lu, Kai-ming
AU  - Lu KM
AD  - Department of Forensic Medicine, Medical College of Soochow University, Suzhou 
      215123, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Fa Yi Xue Za Zhi
JT  - Fa yi xue za zhi
JID - 9426151
RN  - 690G0D6V8H (Ketamine)
RN  - EC 3.4.22.- (Casp3 protein, mouse)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Behavior, Animal/drug effects
MH  - Brain/metabolism/*pathology
MH  - Caspase 3/metabolism
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Forensic Pathology/methods
MH  - Hippocampus/metabolism/pathology
MH  - In Situ Nick-End Labeling
MH  - Injections, Intravenous
MH  - Ketamine/administration & dosage/*poisoning
MH  - Male
MH  - Mice
MH  - Neurons/*drug effects/metabolism/pathology
MH  - Time Factors
EDAT- 2014/01/29 06:00
MHDA- 2015/01/09 06:00
CRDT- 2014/01/29 06:00
PHST- 2014/01/29 06:00 [entrez]
PHST- 2014/01/29 06:00 [pubmed]
PHST- 2015/01/09 06:00 [medline]
PST - ppublish
SO  - Fa Yi Xue Za Zhi. 2013 Oct;29(5):325-9.