PMID- 24467611
OWN - NLM
STAT- MEDLINE
DCOM- 20150317
LR  - 20211021
IS  - 1464-410X (Electronic)
IS  - 1464-4096 (Print)
IS  - 1464-4096 (Linking)
VI  - 114
IP  - 6
DP  - 2014 Dec
TI  - Subtyping of renal cortical neoplasms in fine needle aspiration biopsies using a 
      decision tree based on genomic alterations detected by fluorescence in situ 
      hybridization.
PG  - 881-90
LID - 10.1111/bju.12643 [doi]
AB  - OBJECTIVES: To improve the overall accuracy of diagnosis in needle biopsies of 
      renal masses, especially small renal masses (SRMs), using fluorescence in situ 
      hybridization (FISH), and to develop a renal cortical neoplasm classification 
      decision tree based on genomic alterations detected by FISH. PATIENTS AND 
      METHODS: Ex vivo fine needle aspiration biopsies of 122 resected renal cortical 
      neoplasms were subjected to FISH using a series of seven-probe sets to assess 
      gain or loss of 10 chromosomes and rearrangement of the 11q13 locus. Using 
      specimen (nephrectomy)-histology as the 'gold standard', a genomic 
      aberration-based decision tree was generated to classify specimens. The 
      diagnostic potential of the decision tree was assessed by comparing the 
      FISH-based classification and biopsy histology with specimen histology. RESULTS: 
      Of the 114 biopsies diagnostic by either method, a higher diagnostic yield was 
      achieved by FISH (92 and 96%) than histology alone (82 and 84%) in the 65 
      biopsies from SRMs (<4 cm) and 49 from larger masses, respectively. An optimized 
      decision tree was constructed based on aberrations detected in eight chromosomes, 
      by which the maximum concordance of classification achieved by FISH was 79%, 
      irrespective of mass size. In SRMs, the overall sensitivity of diagnosis by FISH 
      compared with histopathology was higher for benign oncocytoma, was similar for 
      the chromophobe renal cell carcinoma subtype, and was lower for clear-cell and 
      papillary subtypes. The diagnostic accuracy of classification of needle biopsy 
      specimens (from SRMs) increased from 80% obtained by histology alone to 94% when 
      combining histology and FISH. CONCLUSION: The present study suggests that a novel 
      FISH assay developed by us has a role to play in assisting in the yield and 
      accuracy of diagnosis of renal cortical neoplasms in needle biopsies in 
      particular, and can help guide the clinical management of patients with SRMs that 
      were non-diagnostic by histology.
CI  - (c) 2014 The Authors. BJU International published by John Wiley & Sons Ltd on 
      behalf of BJU International.
FAU - Gowrishankar, Banumathy
AU  - Gowrishankar B
AD  - CancerGenetics, Inc., Rutherford, NJ, USA.
FAU - Cahill, Lynnette
AU  - Cahill L
FAU - Arndt, Alexandra E
AU  - Arndt AE
FAU - Al-Ahmadie, Hikmat
AU  - Al-Ahmadie H
FAU - Lin, Oscar
AU  - Lin O
FAU - Chadalavada, Kalyani
AU  - Chadalavada K
FAU - Chaganti, Seeta
AU  - Chaganti S
FAU - Nanjangud, Gouri J
AU  - Nanjangud GJ
FAU - Murty, Vundavalli V
AU  - Murty VV
FAU - Chaganti, Raju S K
AU  - Chaganti RS
FAU - Reuter, Victor E
AU  - Reuter VE
FAU - Houldsworth, Jane
AU  - Houldsworth J
LA  - eng
GR  - P30 CA008748/CA/NCI NIH HHS/United States
GR  - R44 CA134103/CA/NCI NIH HHS/United States
GR  - CA134103/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140715
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
SB  - IM
MH  - Biopsy, Fine-Needle/*methods
MH  - Chromosome Aberrations
MH  - Decision Trees
MH  - Diagnosis, Computer-Assisted/*methods
MH  - Female
MH  - Genomics/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney Neoplasms/*classification/genetics/*surgery
MH  - Male
PMC - PMC4257075
OTO - NOTNLM
OT  - algorithm
OT  - classification
OT  - fine needle aspiration biopsies
OT  - fluorescence in situ hybridization
OT  - oncocytoma
OT  - renal cell carcinoma
EDAT- 2014/01/29 06:00
MHDA- 2015/03/18 06:00
CRDT- 2014/01/29 06:00
PHST- 2014/01/29 06:00 [entrez]
PHST- 2014/01/29 06:00 [pubmed]
PHST- 2015/03/18 06:00 [medline]
AID - 10.1111/bju.12643 [doi]
PST - ppublish
SO  - BJU Int. 2014 Dec;114(6):881-90. doi: 10.1111/bju.12643. Epub 2014 Jul 15.