PMID- 24468291
OWN - NLM
STAT- MEDLINE
DCOM- 20140912
LR  - 20211021
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 11
DP  - 2014 Jan 27
TI  - Adeno-associated virus type 2 preferentially integrates single genome copies with 
      defined breakpoints.
PG  - 15
LID - 10.1186/1743-422X-11-15 [doi]
AB  - BACKGROUND: Adeno-associated virus (AAV) serotype 2 prevalently infects humans 
      and is the only described eukaryotic virus that integrates site-preferentially. 
      In a recent high throughput study, the genome wide distribution of AAV-2 
      integrants was determined using Integrant Capture Sequencing (IC-Seq). Additional 
      insight regarding the integration of AAV-2 into human genomic DNA could be 
      gleaned by low-throughput sequencing of complete viral-chromosomal junctions. 
      FINDINGS: In this study, 140 clones derived from Integrant-Capture Sequencing 
      were sequenced. 100 met sequence inclusion criteria, and of these 39 contained 
      validated junction sequences. These unique sequences were analyzed to investigate 
      the structure and location of viral-chromosomal junctions. CONCLUSIONS: Overall 
      the low-throughput analysis confirmed the genome wide distribution profile 
      gathered through the IC-Seq analysis. We found no unidentifiable sequence 
      inserted at AAV-2 chromosomal junctions. Assessing both left and right ends of 
      the AAV genome, viral breakpoints predominantly occurred in one hairpin of the 
      inverted terminal repeat and AAV genomes were preferentially integrated as single 
      copies.
FAU - Janovitz, Tyler
AU  - Janovitz T
FAU - Sadelain, Michel
AU  - Sadelain M
FAU - Falck-Pedersen, Erik
AU  - Falck-Pedersen E
AD  - Department of Microbiology and Immunology, Weill Medical College of Cornell 
      University, New York, NY 10065, USA. efalckp@med.cornell.edu.
LA  - eng
GR  - R01 AI094050/AI/NIAID NIH HHS/United States
GR  - T32 GM007739/GM/NIGMS NIH HHS/United States
GR  - R01AI094050/AI/NIAID NIH HHS/United States
GR  - T32GM07739/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20140127
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
SB  - IM
MH  - Dependovirus/genetics/*physiology
MH  - Genome, Human
MH  - Humans
MH  - Sequence Analysis, DNA
MH  - *Virus Integration
PMC - PMC3918229
EDAT- 2014/01/29 06:00
MHDA- 2014/09/13 06:00
PMCR- 2014/01/27
CRDT- 2014/01/29 06:00
PHST- 2013/11/01 00:00 [received]
PHST- 2014/01/22 00:00 [accepted]
PHST- 2014/01/29 06:00 [entrez]
PHST- 2014/01/29 06:00 [pubmed]
PHST- 2014/09/13 06:00 [medline]
PHST- 2014/01/27 00:00 [pmc-release]
AID - 1743-422X-11-15 [pii]
AID - 10.1186/1743-422X-11-15 [doi]
PST - epublish
SO  - Virol J. 2014 Jan 27;11:15. doi: 10.1186/1743-422X-11-15.