PMID- 24468349
OWN - NLM
STAT- MEDLINE
DCOM- 20140919
LR  - 20191210
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 120
DP  - 2014 Mar
TI  - Validation and use of a QuEChERS-based gas chromatographic-tandem mass 
      spectrometric method for multiresidue pesticide analysis in blackcurrants 
      including studies of matrix effects and estimation of measurement uncertainty.
PG  - 106-13
LID - S0039-9140(13)00975-2 [pii]
LID - 10.1016/j.talanta.2013.11.087 [doi]
AB  - A triple quadrupole GC-QqQ-MS/MS method was optimized for multiresidue analysis 
      of over 180 pesticides in blackcurrants. The samples were prepared by using a 
      modified quick, easy, cheap, effective, rugged and safe (QuEChERS) analytical 
      protocol. To reduce matrix co-extractives in the final extract, the supernatant 
      was cleaned up by dispersive-solid phase extraction (dispersive-SPE) with a 
      mixture of sorbents: primary secondary amine (PSA), octadecyl (C18) and 
      graphitized carbon black (GCB). The validation results demonstrated fitness for 
      purpose of the streamlined method. The overall recoveries at the three spiking 
      levels of 0.01, 0.05 and 0.2 mg kg(-1) spanned between 70% and 116% (102% on 
      average) with relative standard deviation (RSD) values between 3% and 19% except 
      for chlorothalonil (23%). Response linearity was studied in the range between 
      0.005 and 0.5 mg kg(-1). The matrix effect for each individual compound was 
      evaluated through the study of ratios of the slopes obtained in solvent and 
      blackcurrant matrix. The optimized method provided small matrix effect (<10%) for 
      77% of the compounds, whereas only for 14%, 5% and 4% compounds, the matrix 
      effect was 10-20%, 20-30% and >30%, respectively. Following the application of 
      "top-down" approach, the expanded measurement uncertainty was estimated as being 
      21% on average (coverage factor k=2, confidence level 95%). If compared with 
      samples of other crops, the analyses of blackcurrants revealed a high percentage 
      of exceedance of the legislative maximum residue levels (MRLs), as well as some 
      instances of the detection of pesticides unapproved on this crop.
CI  - (c) 2013 Published by Elsevier B.V.
FAU - Walorczyk, Stanislaw
AU  - Walorczyk S
AD  - Institute of Plant Protection-National Research Institute, Wladyslawa Wegorka 20, 
      60-318 Poznan, Poland. Electronic address: s_walorczyk@tlen.pl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20131206
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Pesticides)
SB  - IM
MH  - Crops, Agricultural/*chemistry
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Limit of Detection
MH  - Pesticides/*analysis
MH  - Ribes/chemistry
MH  - Solid Phase Extraction/*methods
MH  - Tandem Mass Spectrometry/methods
MH  - Uncertainty
OTO - NOTNLM
OT  - Blackcurrant.
OT  - Dispersive solid phase extraction
OT  - Gas chromatography
OT  - Pesticide residue analysis
OT  - Tandem mass spectrometry
OT  - Validation
EDAT- 2014/01/29 06:00
MHDA- 2014/09/23 06:00
CRDT- 2014/01/29 06:00
PHST- 2013/09/11 00:00 [received]
PHST- 2013/11/27 00:00 [revised]
PHST- 2013/11/30 00:00 [accepted]
PHST- 2014/01/29 06:00 [entrez]
PHST- 2014/01/29 06:00 [pubmed]
PHST- 2014/09/23 06:00 [medline]
AID - S0039-9140(13)00975-2 [pii]
AID - 10.1016/j.talanta.2013.11.087 [doi]
PST - ppublish
SO  - Talanta. 2014 Mar;120:106-13. doi: 10.1016/j.talanta.2013.11.087. Epub 2013 Dec 
      6.