PMID- 2447091
OWN - NLM
STAT- MEDLINE
DCOM- 19880225
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 263
IP  - 3
DP  - 1988 Jan 25
TI  - Human transforming growth factor beta.alpha 2-macroglobulin complex is a latent 
      form of transforming growth factor beta.
PG  - 1535-41
AB  - 125I-Labeled human platelet-derived transforming growth factor beta 
      (125I-TGF-beta) and human alpha 2-macroglobulin (alpha 2M) formed a complex as 
      demonstrated by 5% native polyacrylamide gel electrophoresis. The 
      125I-TGF-beta.alpha 2M complex migrated at a position identical to that of the 
      fast migrating form of alpha 2M. Most of the 125I-TGF-beta.alpha 2M complex could 
      be dissociated by acid or urea treatment. When 125I-TGF-beta was incubated with 
      serum, the high molecular weight form of 125I-TGF-beta could be 
      immunoprecipitated by anti-human alpha 2M anti-sera as demonstrated by sodium 
      dodecyl sulfate-polyacrylamide gel electrophoresis. alpha 2M purified from 
      platelet-rich plasma also showed the latent transforming growth factor activity 
      and immunoreactivity of TGF-beta. These results suggest that TGF-beta.alpha 2M 
      complex is a latent form of TGF-beta.
FAU - Huang, S S
AU  - Huang SS
AD  - E. A. Doisy Department of Biochemistry, St. Louis University School of Medicine, 
      Missouri 63104.
FAU - O'Grady, P
AU  - O'Grady P
FAU - Huang, J S
AU  - Huang JS
LA  - eng
GR  - CA 38808/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Macromolecular Substances)
RN  - 0 (Methylamines)
RN  - 0 (Peptides)
RN  - 0 (alpha-Macroglobulins)
RN  - 76057-06-2 (Transforming Growth Factors)
RN  - BSF23SJ79E (methylamine)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Chromatography, Gel
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Macromolecular Substances
MH  - Methylamines/pharmacology
MH  - Peptides/*metabolism
MH  - Transforming Growth Factors
MH  - Trypsin/metabolism
MH  - alpha-Macroglobulins/*metabolism
EDAT- 1988/01/25 00:00
MHDA- 1988/01/25 00:01
CRDT- 1988/01/25 00:00
PHST- 1988/01/25 00:00 [pubmed]
PHST- 1988/01/25 00:01 [medline]
PHST- 1988/01/25 00:00 [entrez]
AID - S0021-9258(19)57337-9 [pii]
PST - ppublish
SO  - J Biol Chem. 1988 Jan 25;263(3):1535-41.