PMID- 2447227
OWN - NLM
STAT- MEDLINE
DCOM- 19880224
LR  - 20061115
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 69 ( Pt 1)
DP  - 1988 Jan
TI  - Measles virus strain-dependent variation in outcome of infection of human blood 
      mononuclear cells.
PG  - 247-52
AB  - Eight measles virus strains including four subacute sclerosing panencephalitis 
      (SSPE) isolates were compared on the basis of their growth characteristics in 
      human peripheral blood mononuclear cells and effect on mitogen-stimulated 
      lymphocyte proliferation. The Edmonston strain virus and some other strains of 
      measles virus replicated in phytohaemagglutinin (PHA)-stimulated lymphocytes and 
      released high titres of infectious virus into the culture medium. Inhibition of 
      DNA synthesis was relatively low at the beginning of lymphocyte proliferation and 
      could be detected only at high multiplicity of infection. Three to 4 days after 
      initiation of proliferation a strong inhibition was seen also in cultures 
      infected with low doses of virus, and was apparently related to the production of 
      high titres of new infectious virus. Some virus strains originally isolated from 
      SSPE patients produced only a small amount of infectious virus in lymphocytes at 
      37 degrees C but had a very strong inhibitory effect on lymphocyte proliferation 
      leading to early cell death. The inhibitory effect was found over a wide range of 
      virus concentrations, but was strictly dose-dependent and no increase in 
      inhibition with low multiplicities of infection could be seen with longer culture 
      times. The amount of interferon induced by different strains varied from 400 to 
      6400 international units/ml but the amount of interferon produced in the cultures 
      did not correlate with the inhibitory effect on proliferation or with the amount 
      of released new infectious virus. The Halle measles strain, originally isolated 
      from an SSPE patient, differed most from the Edmonston strain in its 
      characteristics, and was studied in greater detail. Although only a small amount 
      of infectious virus was produced, the Halle strain had a very strong inhibiting 
      effect on the proliferation of PHA-stimulated lymphocytes, and extensive 
      syncytium formation was seen leading to cell death within 3 days.
FAU - Ilonen, J
AU  - Ilonen J
AD  - Department of Medical Microbiology and Infectious Diseases, Faculty of Medicine, 
      University of Alberta, Edmonton, Canada.
FAU - Salonen, R
AU  - Salonen R
FAU - Marusyk, R
AU  - Marusyk R
FAU - Salmi, A
AU  - Salmi A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Viral)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - DNA, Viral/biosynthesis
MH  - Humans
MH  - Interferons/biosynthesis
MH  - Kinetics
MH  - Leukocytes, Mononuclear/*microbiology
MH  - Lymphocyte Activation
MH  - Lymphocytes/microbiology
MH  - Measles virus/classification/*growth & development
MH  - Subacute Sclerosing Panencephalitis/*microbiology
MH  - Vero Cells
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
AID - 10.1099/0022-1317-69-1-247 [doi]
PST - ppublish
SO  - J Gen Virol. 1988 Jan;69 ( Pt 1):247-52. doi: 10.1099/0022-1317-69-1-247.