PMID- 24473579
OWN - NLM
STAT- MEDLINE
DCOM- 20150109
LR  - 20211021
IS  - 1432-0886 (Electronic)
IS  - 0009-5915 (Linking)
VI  - 123
IP  - 3
DP  - 2014 Jun
TI  - Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus 
      maximus) using FISH with BAC clones.
PG  - 281-91
LID - 10.1007/s00412-014-0452-2 [doi]
AB  - Bacterial artificial chromosomes (BAC) have been widely used for fluorescence in 
      situ hybridization (FISH) mapping of chromosome landmarks in different organisms, 
      including a few in teleosts. In this study, we used BAC-FISH to consolidate the 
      previous genetic and cytogenetic maps of the turbot (Scophthalmus maximus), a 
      commercially important pleuronectiform. The maps consisted of 24 linkage groups 
      (LGs) but only 22 chromosomes. All turbot LGs were assigned to specific 
      chromosomes using BAC probes obtained from a turbot 5x genomic BAC library. It 
      consisted of 46,080 clones with inserts of at least 100 kb and <5 % empty 
      vectors. These BAC probes contained gene-derived or anonymous markers, most of 
      them linked to quantitative trait loci (QTL) related to productive traits. BAC 
      clones were mapped by FISH to unique marker-specific chromosomal positions, which 
      showed a notable concordance with previous genetic mapping data. The two 
      metacentric pairs were cytogenetically assigned to LG2 and LG16, and the 
      nucleolar organizer region (NOR)-bearing pair was assigned to LG15. Double-color 
      FISH assays enabled the consolidation of the turbot genetic map into 22 linkage 
      groups by merging LG8 with LG18 and LG21 with LG24. In this work, a 
      first-generation probe panel of BAC clones anchored to the turbot linkage and 
      cytogenetical map was developed. It is a useful tool for chromosome traceability 
      in turbot, but also relevant in the context of pleuronectiform karyotypes, which 
      often show small hardly identifiable chromosomes. This panel will also be 
      valuable for further integrative genomics of turbot within Pleuronectiformes and 
      teleosts, especially for fine QTL mapping for aquaculture traits, comparative 
      genomics, and whole-genome assembly.
FAU - Taboada, Xoana
AU  - Taboada X
AD  - Departamento de Genetica, Facultad de Biologia, CIBUS, Universidad de Santiago de 
      Compostela, 15782, Campus Vida, Santiago de Compostela, Spain.
FAU - Pansonato-Alves, Jose C
AU  - Pansonato-Alves JC
FAU - Foresti, Fausto
AU  - Foresti F
FAU - Martinez, Paulino
AU  - Martinez P
FAU - Vinas, Ana
AU  - Vinas A
FAU - Pardo, Belen G
AU  - Pardo BG
FAU - Bouza, Carmen
AU  - Bouza C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140129
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Cytogenetic Analysis
MH  - Flatfishes/*genetics
MH  - Genetic Linkage
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Physical Chromosome Mapping
MH  - Quantitative Trait Loci
EDAT- 2014/01/30 06:00
MHDA- 2015/01/13 06:00
CRDT- 2014/01/30 06:00
PHST- 2013/11/04 00:00 [received]
PHST- 2014/01/10 00:00 [accepted]
PHST- 2014/01/09 00:00 [revised]
PHST- 2014/01/30 06:00 [entrez]
PHST- 2014/01/30 06:00 [pubmed]
PHST- 2015/01/13 06:00 [medline]
AID - 10.1007/s00412-014-0452-2 [doi]
PST - ppublish
SO  - Chromosoma. 2014 Jun;123(3):281-91. doi: 10.1007/s00412-014-0452-2. Epub 2014 Jan 
      29.