PMID- 24473785
OWN - NLM
STAT- MEDLINE
DCOM- 20140911
LR  - 20211203
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1109
DP  - 2014
TI  - Molecular methods used for detection of minimal residual disease following 
      hematopoietic stem cell transplantation in myeloid disorders.
PG  - 187-207
LID - 10.1007/978-1-4614-9437-9_11 [doi]
AB  - The monitoring of minimal residual disease (MRD) in patients with acute or 
      chronic myeloid disorders is performed routinely after allogeneic or autologous 
      transplantation. The detection of MRD helps to identify patients who are at high 
      risk for leukemic relapse after transplantation. The most commonly used 
      techniques for MRD detection are qualitative and quantitative PCR methods, 
      fluorescence in situ hybridization (FISH), fluorescence-activated cell sorting 
      (FACS), and cytogenetic analysis, which are often performed complementary in 
      order to assess more precisely MRD. Here we describe the most used sensitive 
      real-time RT-PCR methods for chronic and acute myeloid disorders. Besides 
      protocols for real-time RT-PCR and multiplex RT-PCR procedures for the most 
      common fusion-gene transcripts in acute and chronic myeloid disorders, methods 
      for detections of disease-specific genetic mutated alterations, as NPM1 and FLT3 
      gene length mutations, and aberrantly expressed genes, as WT1 gene transcripts, 
      are described in detail for daily use.
FAU - Elmaagacli, Ahmet H
AU  - Elmaagacli AH
AD  - Department of Bone Marrow Transplantation, University Hospital of Essen, 
      Hufelandstr, Germany.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (NPM1 protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 117896-08-9 (Nucleophosmin)
SB  - IM
MH  - Chromosome Aberrations
MH  - Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - Leukemia, Myeloid/*genetics/*pathology/therapy
MH  - Mutation
MH  - Neoplasm, Residual/*diagnosis/*genetics
MH  - Nucleophosmin
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Polymerase Chain Reaction/*methods
EDAT- 2014/01/30 06:00
MHDA- 2014/09/12 06:00
CRDT- 2014/01/30 06:00
PHST- 2014/01/30 06:00 [entrez]
PHST- 2014/01/30 06:00 [pubmed]
PHST- 2014/09/12 06:00 [medline]
AID - 10.1007/978-1-4614-9437-9_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1109:187-207. doi: 10.1007/978-1-4614-9437-9_11.