PMID- 24477173
OWN - NLM
STAT- MEDLINE
DCOM- 20150219
LR  - 20181202
IS  - 1526-0976 (Electronic)
IS  - 1089-2591 (Linking)
VI  - 18
IP  - 3
DP  - 2014 Jul
TI  - Fluorescence in situ hybridization testing for the diagnosis of high-grade 
      cervical abnormalities: a systematic review.
PG  - 218-27
LID - 10.1097/LGT.0000000000000007 [doi]
AB  - OBJECTIVE: We examined the diagnostic performance of fluorescence in situ 
      hybridization (FISH) tests on cervical cytology for precancerous lesions or 
      cancer on cervical histology. MATERIALS AND METHODS: A search was conducted in 
      MEDLINE, the Cochrane Central Register of Controlled Trials, and Scopus through 
      September 3, 2013. Eleven studies examined FISH tests for telomerase RNA 
      component gene (TERC), myelocytomatosis oncogene (MYC), or human papillomavirus 
      (HPV) type 16 or 18 in samples exhibiting atypical squamous cells of unknown 
      significance (ASC-US) or low-grade squamous intraepithelial lesions (LSIL). None 
      examined HPV-positive, cytologically normal samples. We extracted data on the 
      sensitivity and specificity for high-grade cervical intraepithelial neoplasia 
      (CIN 2+ or CIN 3+). RESULTS: Fluorescence in situ hybridization test probes and 
      thresholds varied across studies. Included populations were convenience samples. 
      Only 1 study testing for TERC specified HPV status. In meta-analysis, FISH for 
      TERC in LSIL (9 studies, 1,082 cases) had a summary sensitivity of 0.76 (95% 
      confidence interval = 0.63-0.85) and a summary specificity of 0.78 (95% 
      confidence interval = 0.57-0.91) for CIN 2+. Fluorescence in situ hybridization 
      for TERC in ASC-US (3 studies, 839 cases) showed sensitivities ranging from 0.75 
      to 1.00 and specificities from 0.87 to 0.93 for CIN 2+. For CIN 3+, sensitivity 
      and specificity appeared similar, although a small number of studies preclude 
      firm conclusions. For FISH tests for HPV, we found only few studies with small 
      sample sizes. CONCLUSIONS: The evidence on FISH testing is limited given the 
      small number of studies for each cytology subgroup and the lack of studies in 
      well-defined screening contexts stratifying participants by HPV status.
FAU - Earley, Amy
AU  - Earley A
AD  - 1Center for Clinical Evidence Synthesis and 2Institute of Clinical Research and 
      Health Policy Study, Tufts Medical Center, Boston, MA; 3Center of Evidence-Based 
      Medicine and 4Department of Health Services Policy & Practice, Program in Public 
      Health, Brown University, Providence, RI; 5Division of Cytogenetics, Department 
      of Pathology and Laboratory Medicine, Tufts Medical Center, Boston, MA; 6Division 
      of Gynecologic Oncology, Department of Obstetrics and Gynecology, Brigham & 
      Women's Hospital, Boston, MA; and 7Division of Nephrology, Department of 
      Medicine, Tufts Medical Center, Boston, MA.
FAU - Lamont, Jenny L
AU  - Lamont JL
FAU - Dahabreh, Issa J
AU  - Dahabreh IJ
FAU - Cowan, Janet
AU  - Cowan J
FAU - Feldman, Sarah
AU  - Feldman S
FAU - Uhlig, Katrin
AU  - Uhlig K
LA  - eng
GR  - HHSA 290 2007 10055 I/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PT  - Systematic Review
PL  - United States
TA  - J Low Genit Tract Dis
JT  - Journal of lower genital tract disease
JID - 9704963
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Female
MH  - *Genes, myc
MH  - Human papillomavirus 16/genetics/*isolation & purification
MH  - Human papillomavirus 18/genetics/*isolation & purification
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - RNA/*analysis/genetics
MH  - Sensitivity and Specificity
MH  - Telomerase/*analysis/genetics
MH  - Uterine Cervical Neoplasms/*diagnosis
EDAT- 2014/01/31 06:00
MHDA- 2015/02/20 06:00
CRDT- 2014/01/31 06:00
PHST- 2014/01/31 06:00 [entrez]
PHST- 2014/01/31 06:00 [pubmed]
PHST- 2015/02/20 06:00 [medline]
AID - 10.1097/LGT.0000000000000007 [doi]
PST - ppublish
SO  - J Low Genit Tract Dis. 2014 Jul;18(3):218-27. doi: 10.1097/LGT.0000000000000007.