PMID- 24478136
OWN - NLM
STAT- MEDLINE
DCOM- 20141023
LR  - 20161125
IS  - 1521-3773 (Electronic)
IS  - 1433-7851 (Linking)
VI  - 53
IP  - 9
DP  - 2014 Feb 24
TI  - Quantitative analysis of location- and sequence-dependent deamination by APOBEC3G 
      using real-time NMR spectroscopy.
PG  - 2349-52
LID - 10.1002/anie.201309940 [doi]
AB  - The human antiretroviral factor APOBEC3G (A3G) deaminates the newly synthesized 
      minus strand of the human immunodeficiency virus 1 (HIV-1), which results in the 
      abolition of the infectivity of virus-infectivity-factor (Vif)-deficient HIV-1 
      strains.1-6 A unique property of A3G is that it deaminates a CCC hot spot that is 
      located close to the 5' end more effectively than one that is less close to the 
      5' end. However, the mechanism of this process is elusive as it includes 
      nonspecific binding of A3G to DNA and sliding of A3G along the DNA strand. 
      Therefore, this process cannot be analyzed by existing methods using the 
      Michaelis-Menten theory. A new real-time NMR method has been developed to examine 
      the nonspecific binding and the sliding processes explicitly, and it was applied 
      to the analysis of the deamination by A3G. As a result, the location-dependent 
      deamination can be explained by a difference in the catalytic rates that depend 
      on the direction of the approach of A3G to the target cytidine. Real-time NMR 
      experiments also showed that A3G deaminates CCCC tandem hotspots with little 
      redundancy, which suggests that A3G efficiently mutates many CCC hotspots that 
      are scattered throughout the HIV-1 genome.
CI  - Copyright (c) 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Furukawa, Ayako
AU  - Furukawa A
AD  - Institute of Advanced Energy, Graduate School of Energy Science, Kyoto 
      University, Gokasho, Uji, Kyoto 611-0011 (Japan).
FAU - Sugase, Kenji
AU  - Sugase K
FAU - Morishita, Ryo
AU  - Morishita R
FAU - Nagata, Takashi
AU  - Nagata T
FAU - Kodaki, Tsutomu
AU  - Kodaki T
FAU - Takaori-Kondo, Akifumi
AU  - Takaori-Kondo A
FAU - Ryo, Akihide
AU  - Ryo A
FAU - Katahira, Masato
AU  - Katahira M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140129
PL  - Germany
TA  - Angew Chem Int Ed Engl
JT  - Angewandte Chemie (International ed. in English)
JID - 0370543
RN  - 0 (DNA, Single-Stranded)
RN  - EC 3.5.4.5 (APOBEC-3G Deaminase)
RN  - EC 3.5.4.5 (APOBEC3G protein, human)
RN  - EC 3.5.4.5 (Cytidine Deaminase)
SB  - IM
MH  - APOBEC-3G Deaminase
MH  - Base Sequence
MH  - Cytidine Deaminase/*metabolism
MH  - DNA, Single-Stranded/chemistry/*metabolism
MH  - Deamination
MH  - HIV Infections/metabolism
MH  - HIV-1/physiology
MH  - Host-Pathogen Interactions
MH  - Humans
MH  - Nuclear Magnetic Resonance, Biomolecular/*methods
MH  - Protein Binding
OTO - NOTNLM
OT  - DNA
OT  - NMR spectroscopy
OT  - deamination
OT  - enzyme kinetics
OT  - quantitative analysis
EDAT- 2014/01/31 06:00
MHDA- 2014/10/24 06:00
CRDT- 2014/01/31 06:00
PHST- 2013/11/15 00:00 [received]
PHST- 2014/01/31 06:00 [entrez]
PHST- 2014/01/31 06:00 [pubmed]
PHST- 2014/10/24 06:00 [medline]
AID - 10.1002/anie.201309940 [doi]
PST - ppublish
SO  - Angew Chem Int Ed Engl. 2014 Feb 24;53(9):2349-52. doi: 10.1002/anie.201309940. 
      Epub 2014 Jan 29.