PMID- 24478487
OWN - NLM
STAT- MEDLINE
DCOM- 20140915
LR  - 20211021
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 52
IP  - 2
DP  - 2014 Feb
TI  - The majority of a collection of U.S. endocarditis Enterococcus faecalis isolates 
      obtained from 1974 to 2004 lack capsular genes and belong to diverse, 
      non-hospital-associated lineages.
PG  - 549-56
LID - 10.1128/JCM.02763-13 [doi]
AB  - Eighty-one endocarditis-derived Enterococcus faecalis isolates that were 
      collected from individual patients in the United States between 1974 and 2004 
      were sequence typed and analyzed for the presence of various genes, including 
      some previously associated with virulence. Overall, using our previously 
      described trilocus sequence typing (TLST), 44 different sequence types (STs) were 
      found within this collection; 26 isolates were singletons (a unique TLST sequence 
      type [ST(T)]), some ST(T)s contained multiple isolates (up to 6 isolates), and 
      16% of the isolates (13 isolates) could be grouped by additional sequence typing 
      into clonal cluster 21 (CC21). Of note, only four isolates (7%) of the 56 whose 
      multilocus sequence types were determined were found to belong to one of the 
      previously described hospital-associated clonal clusters CC2 and CC9, and only 
      15% and 37% of all isolates had high-level resistance to gentamicin and 
      streptomycin, respectively, including 10% that were resistant to both. We also 
      found that 64% of the isolates lacked the genes for production of capsule 
      polysaccharide, which has been proposed to enhance the pathogenic potential of 
      the hospital-associated clonal clusters. In summary, while our collection is not 
      a random sample of cases of E. faecalis endocarditis, these results indicate that 
      nonencapsulated strains belonging to non-hospital-associated lineages were 
      predominant among endocarditis E. faecalis isolates recovered during this time 
      period.
FAU - Chowdhury, Shahreen A
AU  - Chowdhury SA
AD  - Center for the Study of Emerging and Re-emerging Pathogens, Division of 
      Infectious Diseases, University of Texas Medical School at Houston, Houston, 
      Texas, USA.
FAU - Nallapareddy, Sreedhar R
AU  - Nallapareddy SR
FAU - Arias, Cesar A
AU  - Arias CA
FAU - Murray, Barbara E
AU  - Murray BE
LA  - eng
GR  - R01 AI047923/AI/NIAID NIH HHS/United States
GR  - R01 AI093749/AI/NIAID NIH HHS/United States
GR  - R37 AI047923/AI/NIAID NIH HHS/United States
GR  - R37/R01 AI47923/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20131204
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Gentamicins)
RN  - Y45QSO73OB (Streptomycin)
SB  - IM
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacterial Capsules/*genetics
MH  - Cluster Analysis
MH  - Drug Resistance, Bacterial
MH  - Endocarditis, Bacterial/epidemiology/*microbiology
MH  - Enterococcus faecalis/*classification/genetics/*isolation & purification
MH  - *Genes, Bacterial
MH  - Genotype
MH  - Gentamicins/pharmacology
MH  - Gram-Positive Bacterial Infections/epidemiology/*microbiology
MH  - Humans
MH  - Multilocus Sequence Typing
MH  - Streptomycin/pharmacology
MH  - United States/epidemiology
PMC - PMC3911312
EDAT- 2014/01/31 06:00
MHDA- 2014/09/16 06:00
PMCR- 2014/08/01
CRDT- 2014/01/31 06:00
PHST- 2014/01/31 06:00 [entrez]
PHST- 2014/01/31 06:00 [pubmed]
PHST- 2014/09/16 06:00 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - JCM.02763-13 [pii]
AID - 02763-13 [pii]
AID - 10.1128/JCM.02763-13 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2014 Feb;52(2):549-56. doi: 10.1128/JCM.02763-13. Epub 2013 Dec 
      4.