PMID- 24480801
OWN - NLM
STAT- MEDLINE
DCOM- 20140925
LR  - 20211021
IS  - 2092-6413 (Electronic)
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 46
IP  - 1
DP  - 2014 Jan 31
TI  - Regulation of anti-inflammatory cytokines IL-10 and TGF-beta in mouse dendritic 
      cells through treatment with Clonorchis sinensis crude antigen.
PG  - e74
LID - 10.1038/emm.2013.144 [doi]
AB  - Dendritic cells (DCs), which are regarded as the most potent antigen-presenting 
      cells, are involved in innate and adaptive immunity. Upon uptake of pathogens, 
      DCs express cell surface markers and secrete cytokines. In this study, we 
      analyzed production of cytokines and found that interleukin (IL)-10 and 
      transforming growth factor (TGF)-beta production significantly increased in bone 
      marrow-derived DCs and a mouse DC line, DC2.4, after treatment with crude antigen 
      (CA) from liver fluke, Clonorchis sinensis. However, expression patterns of 
      several activation molecules did not change. In addition, following treatment of 
      DC2.4 cells with antigen from the lung fluke, Paragonimus westermani, production 
      of IL-10 and TGF-beta significantly increased compared with groups treated with 
      other parasite antigens, Spirometra erinacei plerocercoid CA and Echinococcus 
      granulosus hydatid cystic fluid. We also found that treatment of DC2.4 cells with 
      C. sinensis CA resulted in rapid and significant phosphorylation of extracellular 
      signal-regulated kinase 1/2, a mitogen-activated protein kinase. Following 
      treatment of DC2.4 cells with C. sinensis CA, treatment with an inhibitor 
      specific to an extracellular signal-regulated kinase inhibited production of 
      IL-10 and TGF-beta. Our results suggest that CA from C. sinensis has a role in the 
      anti-inflammatory function of DC cells by inducing IL-10 and TGF-beta through 
      activation of extracellular signal-regulated kinase 1/2.
FAU - Jin, Yan
AU  - Jin Y
AD  - Department of Parasitology and Tropical Medicine, Seoul National University 
      College of Medicine and Institute of Endemic Diseases, Seoul National University 
      Medical Research Center, Seoul 110-799, Korea.
FAU - Wi, Hae Joo
AU  - Wi HJ
AD  - Department of Parasitology and Tropical Medicine, Seoul National University 
      College of Medicine and Institute of Endemic Diseases, Seoul National University 
      Medical Research Center, Seoul 110-799, Korea.
FAU - Choi, Min-Ho
AU  - Choi MH
AD  - Department of Parasitology and Tropical Medicine, Seoul National University 
      College of Medicine and Institute of Endemic Diseases, Seoul National University 
      Medical Research Center, Seoul 110-799, Korea.
FAU - Hong, Sung-Tae
AU  - Hong ST
AD  - Department of Parasitology and Tropical Medicine, Seoul National University 
      College of Medicine and Institute of Endemic Diseases, Seoul National University 
      Medical Research Center, Seoul 110-799, Korea.
FAU - Bae, Young Mee
AU  - Bae YM
AD  - Department of Parasitology and Tropical Medicine, Seoul National University 
      College of Medicine and Institute of Endemic Diseases, Seoul National University 
      Medical Research Center, Seoul 110-799, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140131
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (Antigens, Helminth)
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Antigens, Helminth/*pharmacology
MH  - Cells, Cultured
MH  - Clonorchis sinensis/*immunology
MH  - Dendritic Cells/drug effects/*metabolism
MH  - Interleukin-10/genetics/*metabolism
MH  - MAP Kinase Signaling System
MH  - Mice
MH  - Transforming Growth Factor beta/genetics/*metabolism
PMC - PMC3909892
EDAT- 2014/02/01 06:00
MHDA- 2014/09/26 06:00
PMCR- 2014/01/01
CRDT- 2014/02/01 06:00
PHST- 2013/06/21 00:00 [received]
PHST- 2013/10/23 00:00 [revised]
PHST- 2013/10/24 00:00 [accepted]
PHST- 2014/02/01 06:00 [entrez]
PHST- 2014/02/01 06:00 [pubmed]
PHST- 2014/09/26 06:00 [medline]
PHST- 2014/01/01 00:00 [pmc-release]
AID - emm2013144 [pii]
AID - 10.1038/emm.2013.144 [doi]
PST - epublish
SO  - Exp Mol Med. 2014 Jan 31;46(1):e74. doi: 10.1038/emm.2013.144.