PMID- 24481225
OWN - NLM
STAT- MEDLINE
DCOM- 20160718
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 33
IP  - 1
DP  - 2014
TI  - Decreased intracellular pH induced by cariporide differentially contributes to 
      human umbilical cord-derived mesenchymal stem cells differentiation.
PG  - 185-94
LID - 10.1159/000356661 [doi]
AB  - BACKGROUND/AIMS: Na+/H+ exchanger 1 (NHE1) is an important regulator of 
      intracellular pH (pHi). High pHi is required for cell proliferation and 
      differentiation. Our previous study has proven that the pHi of mesenchymal stem 
      cells is higher than that of normal differentiated cells and similar to tumor 
      cells. NHE1 is highly expressed in both mesenchymal stem cells and tumor cells. 
      Targeted inhibition of NHE1 could induce differentiation of K562 leukemia cells. 
      In the present paper we explored whether inhibition of NHE1 could induce 
      differentiation of mesenchymal stem cells. METHODS: MSCs were obtained from human 
      umbilical cord and both the surface phenotype and functional characteristics were 
      analyzed. Selective NHE1 inhibitor cariporide was used to treat human umbilical 
      cord-derived mesenchymal stem cells (hUC-MSCs). The pHi and the differentiation 
      of hUC-MSCs were compared upon cariporide treatment. The putative signaling 
      pathway involved was also explored. RESULTS: The pHi of hUC-MSCs was decreased 
      upon cariporide treatment. Cariporide up-regulated the osteogenic differentiation 
      of hUC-MSCs while the adipogenic differentiation was not affected. For osteogenic 
      differentiation, beta-catenin expression was up-regulated upon cariporide treatment. 
      CONCLUSION: Decreased pHi induced by cariporide differentially contributes to 
      hUC-MSCs differentiation.
FAU - Gao, Wei
AU  - Gao W
AD  - State key Laboratory of Experimental Hematology, Institute of Hematology and 
      Hospital of Blood Diseases, Chinese Academy of Medical Sciences and Peking Union 
      Medical College, Tianjin, China.
FAU - Zhang, Hairui
AU  - Zhang H
FAU - Chang, Guoqiang
AU  - Chang G
FAU - Xie, Zhenqing
AU  - Xie Z
FAU - Wang, Hanyu
AU  - Wang H
FAU - Ma, Li
AU  - Ma L
FAU - Han, Zhongchao
AU  - Han Z
FAU - Li, Qinghua
AU  - Li Q
FAU - Pang, Tianxiang
AU  - Pang T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140124
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Cation Transport Proteins)
RN  - 0 (Guanidines)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (SLC9A1 protein, human)
RN  - 0 (Sodium-Hydrogen Exchanger 1)
RN  - 0 (Sodium-Hydrogen Exchangers)
RN  - 0 (Sulfones)
RN  - 0 (beta Catenin)
RN  - 7E3392891K (cariporide)
SB  - IM
MH  - Adipogenesis/drug effects
MH  - Cation Transport Proteins/metabolism
MH  - Cell Death/drug effects
MH  - Cell Differentiation/*drug effects
MH  - Gene Knockdown Techniques
MH  - Guanidines/*pharmacology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Intracellular Space/drug effects/*metabolism
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - Osteogenesis/drug effects
MH  - Phenotype
MH  - RNA, Small Interfering/metabolism
MH  - Sodium-Hydrogen Exchanger 1
MH  - Sodium-Hydrogen Exchangers/metabolism
MH  - Sulfones/*pharmacology
MH  - Umbilical Cord/*cytology
MH  - Up-Regulation/drug effects
MH  - beta Catenin/metabolism
EDAT- 2014/02/01 06:00
MHDA- 2016/07/19 06:00
CRDT- 2014/02/01 06:00
PHST- 2013/12/18 00:00 [accepted]
PHST- 2014/02/01 06:00 [entrez]
PHST- 2014/02/01 06:00 [pubmed]
PHST- 2016/07/19 06:00 [medline]
AID - 000356661 [pii]
AID - 10.1159/000356661 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2014;33(1):185-94. doi: 10.1159/000356661. Epub 2014 Jan 
      24.