PMID- 24481743
OWN - NLM
STAT- MEDLINE
DCOM- 20150102
LR  - 20211021
IS  - 1993-0402 (Electronic)
IS  - 1672-0415 (Linking)
VI  - 20
IP  - 5
DP  - 2014 May
TI  - Zhuanggu Jianxi Decoction () limits interleukin-1 beta-induced degeneration 
      chondrocytes via the caveolin-p38 MAPK signal pathway.
PG  - 353-9
LID - 10.1007/s11655-014-1801-y [doi]
AB  - OBJECTIVE: To evaluate the effect of Zhuanggu Jianxi Decoction (, ZGJXD) on 
      interleukin-1 beta (IL-1 beta)-induced degeneration of chondrocytes (CDs) as well as 
      the activation of caveolin-p38 mitogen-activated protein kinase (MAPK) signal 
      pathway, investigating the possible molecular mechanism that ZGJXD treats 
      osteoarthritis. METHODS: Serum pharmacology was applied in the present study, 
      where ZGJXD was orally administrated to New Zealand rabbits and then ZGJXD 
      containing serum (ZGJXD-S) was collected for following in vitro experiments. CDs 
      were isolated aseptically from New Zealand rabbits and then cultured in vitro. 
      Upon IL-1 beta stimulation, the degeneration of CDs was verified by inverted 
      microscope, toluidine blue stain and type II collagen immunocytochemistry. After 
      IL-1 beta-stimulated CDs were intervened with blank control serum, ZGJXD-S, together 
      with or without SB203580 (a specific inhibitor of p38 MAPK) for 48 h, caveolin-1 
      protein expression and the phosphorylation level of p38 were determined by 
      Western blotting, and the mRNA expression of IL-1 beta, tumor necrosis factor alpha 
      (TNF-alpha), matrix metalloproteinase 3 (MMP-3) and MMP-13 were examined by real-time 
      polymerase chain reaction. RESULTS: IL-1 beta stimulation induced degeneration of 
      CDs, increased caveolin-1 expression and p38 phosphorylation, up-regulated the 
      mRNA level of IL-1 beta, TNF-alpha, MMP-3 and MMP-13. However, the IL-1 beta-induced 
      activation of caveolin-p38 signaling and alteration in the expression of p38 
      downstream target genes were suppressed by ZGJXD-S and/or SB203580 in CDs. 
      CONCLUSION: ZGJXD can prevent CDs degeneration via inhibition of caveolin-p38 
      MAPK signal pathway, which might be one of the mechanisms that ZGJXD treats 
      osteoarthritis.
FAU - Yan, Hu
AU  - Yan H
AD  - Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China.
FAU - Su, You-xin
AU  - Su YX
FAU - Lin, Xue-yi
AU  - Lin XY
FAU - Chen, Bao-jun
AU  - Chen BJ
FAU - Zhang, Qing
AU  - Zhang Q
FAU - Zhang, Zi-yi
AU  - Zhang ZY
FAU - Wang, Yi-ru
AU  - Wang YR
FAU - Li, Ya-nan
AU  - Li YN
FAU - Lu, Mei-li
AU  - Lu ML
FAU - He, Zhen
AU  - He Z
FAU - Sheng, Lu
AU  - Sheng L
FAU - Wang, Wen-ting
AU  - Wang WT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140130
PL  - China
TA  - Chin J Integr Med
JT  - Chinese journal of integrative medicine
JID - 101181180
RN  - 0 (Caveolins)
RN  - 0 (DNA Primers)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Interleukin-1beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (zhuanggu jianxi)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - Caveolins/*metabolism
MH  - Chondrocytes/*drug effects/enzymology/metabolism
MH  - DNA Primers
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Gene Expression Profiling
MH  - Interleukin-1beta/*physiology
MH  - MAP Kinase Signaling System
MH  - Male
MH  - RNA, Messenger/genetics
MH  - Rabbits
MH  - p38 Mitogen-Activated Protein Kinases/genetics/*metabolism
EDAT- 2014/02/01 06:00
MHDA- 2015/01/03 06:00
CRDT- 2014/02/01 06:00
PHST- 2013/06/13 00:00 [received]
PHST- 2014/02/01 06:00 [entrez]
PHST- 2014/02/01 06:00 [pubmed]
PHST- 2015/01/03 06:00 [medline]
AID - 10.1007/s11655-014-1801-y [doi]
PST - ppublish
SO  - Chin J Integr Med. 2014 May;20(5):353-9. doi: 10.1007/s11655-014-1801-y. Epub 
      2014 Jan 30.