PMID- 24482230
OWN - NLM
STAT- MEDLINE
DCOM- 20140624
LR  - 20211021
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 289
IP  - 10
DP  - 2014 Mar 7
TI  - Structural insights into the interaction between a potent anti-inflammatory 
      protein, viral CC chemokine inhibitor (vCCI), and the human CC chemokine, 
      Eotaxin-1.
PG  - 6592-6603
LID - S0021-9258(20)44478-3 [pii]
LID - 10.1074/jbc.M113.538991 [doi]
AB  - Chemokines play important roles in the immune system, not only recruiting 
      leukocytes to the site of infection and inflammation but also guiding cell homing 
      and cell development. The soluble poxvirus-encoded protein viral CC chemokine 
      inhibitor (vCCI), a CC chemokine inhibitor, can bind to human CC chemokines 
      tightly to impair the host immune defense. This protein has no known homologs in 
      eukaryotes and may represent a potent method to stop inflammation. Previously, 
      our structure of the vCCI.MIP-1beta (macrophage inflammatory protein-1beta) complex 
      indicated that vCCI uses negatively charged residues in beta-sheet II to interact 
      with positively charged residues in the MIP-1beta N terminus, 20s region and 40s 
      loop. However, the interactions between vCCI and other CC chemokines have not yet 
      been fully explored. Here, we used NMR and fluorescence anisotropy to study the 
      interaction between vCCI and eotaxin-1 (CCL11), a CC chemokine that is an 
      important factor in the asthma response. NMR results reveal that the binding 
      pattern is very similar to the vCCI.MIP-1beta complex and suggest that electrostatic 
      interactions provide a major contribution to binding. Fluorescence anisotropy 
      results on variants of eotaxin-1 further confirm the critical roles of the 
      charged residues in eotaxin-1. In addition, the binding affinity between vCCI and 
      other wild type CC chemokines, MCP-1 (monocyte chemoattractant protein-1), 
      MIP-1beta, and RANTES (regulated on activation normal T cell expressed and 
      secreted), were determined as 1.1, 1.2, and 0.22 nm, respectively. To our 
      knowledge, this is the first work quantitatively measuring the binding affinity 
      between vCCI and multiple CC chemokines.
FAU - Kuo, Nai-Wei
AU  - Kuo NW
AD  - Molecular Cell Biology, University of California, Merced, California 95343.
FAU - Gao, Yong-Guang
AU  - Gao YG
AD  - Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological 
      Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
FAU - Schill, Megan S
AU  - Schill MS
AD  - Molecular Cell Biology, University of California, Merced, California 95343.
FAU - Isern, Nancy
AU  - Isern N
AD  - High Field NMR Facility, William R. Wiley Environmental Molecular Sciences 
      Laboratory, Richland, Washington 99352.
FAU - Dupureur, Cynthia M
AU  - Dupureur CM
AD  - Department of Chemistry and Biochemistry, University of Missouri, St. Louis, 
      Missouri 63121.
FAU - LiWang, Patricia J
AU  - LiWang PJ
AD  - Molecular Cell Biology, University of California, Merced, California 95343. 
      Electronic address: pliwang@ucmerced.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20140130
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (CCI protein, Cowpox virus)
RN  - 0 (CCL11 protein, human)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCL4 protein, human)
RN  - 0 (Chemokine CCL11)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Viral Proteins)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Chemokine CCL11/chemistry/genetics/*immunology
MH  - Chemokine CCL2/chemistry/immunology
MH  - Chemokine CCL4/chemistry/immunology
MH  - Chemokine CCL5/chemistry/immunology
MH  - Humans
MH  - Inflammation/immunology
MH  - Molecular Sequence Data
MH  - Nuclear Magnetic Resonance, Biomolecular
MH  - Protein Binding/immunology
MH  - Protein Structure, Secondary
MH  - Viral Proteins/chemistry/*immunology
MH  - Virulence Factors/chemistry/*immunology
PMC - PMC3945322
OTO - NOTNLM
OT  - Anti-inflammatory Protein
OT  - Biophysics
OT  - Chemokine-binding Protein
OT  - Chemokines
OT  - Eotaxin
OT  - Fluorescence Anisotropy
OT  - Molecular Docking
OT  - NMR
OT  - Protein-Protein Interactions
OT  - vCCI
EDAT- 2014/02/01 06:00
MHDA- 2014/06/25 06:00
PMCR- 2015/03/07
CRDT- 2014/02/01 06:00
PHST- 2014/02/01 06:00 [entrez]
PHST- 2014/02/01 06:00 [pubmed]
PHST- 2014/06/25 06:00 [medline]
PHST- 2015/03/07 00:00 [pmc-release]
AID - S0021-9258(20)44478-3 [pii]
AID - M113.538991 [pii]
AID - 10.1074/jbc.M113.538991 [doi]
PST - ppublish
SO  - J Biol Chem. 2014 Mar 7;289(10):6592-6603. doi: 10.1074/jbc.M113.538991. Epub 
      2014 Jan 30.