PMID- 24485403
OWN - NLM
STAT- MEDLINE
DCOM- 20140512
LR  - 20140325
IS  - 2210-7762 (Print)
VI  - 207
IP  - 1-2
DP  - 2014 Jan-Feb
TI  - Modified cIg-FISH protocol for multiple myeloma in routine cytogenetic laboratory 
      practice.
PG  - 31-4
LID - S2210-7762(13)00168-3 [pii]
LID - 10.1016/j.cancergen.2013.12.001 [doi]
AB  - The International Myeloma Working Group recommends that fluorescence in situ 
      hybridization (FISH) be performed on specifically identified plasma cells (PC). 
      This is because chromosomal abnormalities are not frequently detected by 
      traditional karyotyping due to the low proliferative rate of PC in multiple 
      myeloma (MM). Conventional FISH enhances the sensitivity but lacks the 
      specificity, as it does not distinguish PC from other hematopoetic cells. To 
      fulfill this recommendation, PC need to be selected either by flow cytometry or 
      immunomagnetic bead-based PC sorting or by concomitant labeling of the 
      cytoplasmic immunoglobulin light chain, which allows for unambiguous 
      identification. These techniques require expertise, time, and funding and are not 
      easily incorporated into the routine workflow of the cytogenetic laboratory. We 
      have modified and refined the technique using fixed cell pellets to achieve 
      nicely separated and easily identifiable PC. With immunostaining and subsequent 
      FISH (i.e., cytoplasmic immunoglobulin FISH, cIg-FISH), this technique can be 
      easily incorporated into every cytogenetic laboratory. Twenty samples from 
      patients with MM were subjected to routine FISH, cIg-FISH, and chromosomal 
      karyotyping and the results were compared. Three FISH probes, which enabled 
      detection of the t(4;14), t(14;16) and deletion of TP53, were used to validate 
      this modified technique successfully.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Gole, Leena
AU  - Gole L
AD  - Department of Laboratory Medicine, National University Health Systems, Singapore. 
      Electronic address: leena_gole@nuhs.edu.sg.
FAU - Lin, Adeline
AU  - Lin A
AD  - Department of Haematology Oncology, National Cancer Institute of Singapore, 
      Singapore.
FAU - Chua, Constance
AU  - Chua C
AD  - Department of Laboratory Medicine, National University Health Systems, Singapore.
FAU - Chng, Wee Joo
AU  - Chng WJ
AD  - Department of Haematology Oncology, National Cancer Institute of Singapore, 
      Singapore.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131227
PL  - United States
TA  - Cancer Genet
JT  - Cancer genetics
JID - 101539150
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Bone Marrow/pathology
MH  - Cell Proliferation
MH  - Cell Separation
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 14/ultrastructure
MH  - Chromosomes, Human, Pair 16/ultrastructure
MH  - Chromosomes, Human, Pair 4/ultrastructure
MH  - Flow Cytometry
MH  - Genes, p53
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics/immunology
MH  - Oligonucleotide Probes/genetics
MH  - Plasma Cells/*cytology
OTO - NOTNLM
OT  - Multiple myeloma
OT  - cytogenetic analysis
OT  - cytoplasmic immunoglobulin fluorescence in situ hybridization (cIg-FISH)
OT  - plasma cell
EDAT- 2014/02/04 06:00
MHDA- 2014/05/13 06:00
CRDT- 2014/02/04 06:00
PHST- 2013/10/21 00:00 [received]
PHST- 2013/11/29 00:00 [revised]
PHST- 2013/12/16 00:00 [accepted]
PHST- 2014/02/04 06:00 [entrez]
PHST- 2014/02/04 06:00 [pubmed]
PHST- 2014/05/13 06:00 [medline]
AID - S2210-7762(13)00168-3 [pii]
AID - 10.1016/j.cancergen.2013.12.001 [doi]
PST - ppublish
SO  - Cancer Genet. 2014 Jan-Feb;207(1-2):31-4. doi: 10.1016/j.cancergen.2013.12.001. 
      Epub 2013 Dec 27.