PMID- 24489995
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140203
LR  - 20211021
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 6
IP  - 2
DP  - 2014
TI  - Nuclear localization of catalytically active MMP-2 in endothelial cells and 
      neurons.
PG  - 155-62
AB  - From microscopic organelles and sub-cellular domains to the level of whole 
      tissues, organs, and body parts, living organisms must continuously maintain and 
      renovate structural components. Matrix metalloproteinases (MMPs) comprise a 
      family of over two dozen Zn-dependent endopeptidases thought to be primary 
      effectors of extracellular tissue renewal and remodeling processes. Endogenous 
      inhibitors, particularly the tissue inhibitors of MMPs (TIMPs), counteract MMP-2 
      proteolytic activity, but also participate in conversion of several pro-MMPs to 
      proteolytically active forms. Numerous pathologies are characterized by 
      imbalances in activities of MMPs relative to TIMPs. MMPs are synthesized and 
      stored in cytoplasmic domains prior to secretion or expression in cell 
      surface-associated form. Several proteases have been identified in cell nuclei, 
      but their functions, regulation, and substrates remain largely unknown. Here we 
      showed that the catalytically active gelatinase MMP-2 is expressed in nuclei of 
      endothelial cells and neurons, but not in glial or Schwannoma cell lines, in a 
      pattern resembling nuclear speckles, and colocalizes with TIMP-1.
FAU - Sinha, Satyesh K
AU  - Sinha SK
AD  - Cardiometabolic Research Unit, Division of Endocrinology, Metabolism and 
      Molecular Medicine, Charles R. Drew University of Medicine and Science Los 
      Angeles, CA, USA.
FAU - Asotra, Kamlesh
AU  - Asotra K
AD  - Cedars-Sinai Medical Center Los Angeles, California.
FAU - Uzui, Hiroyasu
AU  - Uzui H
AD  - Cedars-Sinai Medical Center Los Angeles, California.
FAU - Nagwani, Santosh
AU  - Nagwani S
AD  - Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University 
      Varanasi, India.
FAU - Mishra, Vivek
AU  - Mishra V
AD  - Molecular Biology Unit, Institute of Medical Sciences, Banaras Hindu University 
      Varanasi, India.
FAU - Rajavashisth, Tripathi B
AU  - Rajavashisth TB
AD  - Cardiometabolic Research Unit, Division of Endocrinology, Metabolism and 
      Molecular Medicine, Charles R. Drew University of Medicine and Science Los 
      Angeles, CA, USA ; Molecular Biology Unit, Institute of Medical Sciences, Banaras 
      Hindu University Varanasi, India ; Omics Biotechnology, Inc. Lawndale, CA, USA.
LA  - eng
GR  - R01 HL051980/HL/NHLBI NIH HHS/United States
GR  - R29 HL051980/HL/NHLBI NIH HHS/United States
GR  - S21 MD000103/MD/NIMHD NIH HHS/United States
GR  - U54 MD007598/MD/NIMHD NIH HHS/United States
PT  - Journal Article
DEP - 20140115
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC3902226
OTO - NOTNLM
OT  - Nuclear localization
OT  - catalytically active MMP-2
OT  - endothelial cells
OT  - neurons
EDAT- 2014/02/04 06:00
MHDA- 2014/02/04 06:01
PMCR- 2014/01/15
CRDT- 2014/02/04 06:00
PHST- 2013/11/26 00:00 [received]
PHST- 2013/12/31 00:00 [accepted]
PHST- 2014/02/04 06:00 [entrez]
PHST- 2014/02/04 06:00 [pubmed]
PHST- 2014/02/04 06:01 [medline]
PHST- 2014/01/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2014 Jan 15;6(2):155-62. eCollection 2014.