PMID- 24490505
OWN - NLM
STAT- MEDLINE
DCOM- 20140829
LR  - 20140204
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 44
IP  - 6
DP  - 2013 Nov
TI  - [Effect of human umbilici mesenchymal stromal cells implantation on the BDNF 
      expression in diabetic foot rats].
PG  - 931-4
AB  - OBJECTITVE: To investigate the effect of human umbilici mesenchymal cells 
      (HUMSCs) implantation on the brain derived neurotrophic factor (BDNF) expression 
      in diabetic foot rats. METHODS: SD rats were divided into three groups (n = 12): 
      normal group, diadetic foot model group and HUMSC treatment group. Diabetic foot 
      model in rats was established, then prepared HUMSC were implanted on the diabetic 
      foot ulcers in rats, and control ones were administrated with saline only. The 
      area of ulceration, sensory function, BDNF expression and its localization were 
      determined by using morphology, physiological function measurement, RT-PCR and 
      immunohistochemistry assay. RESULT: Siglificantly decreased area of ulceration in 
      diabetic foot rats of HUMSC implantation group was observed. This was 
      simultaneously companied with the sensory function improvement (P < 0.05). RT-PCR 
      showed that BDNF mRNA expression was significantly up regulated (P < 0.05). BDNF 
      immunstaining was located in epithelia tissue and the protein level of BDNF was 
      markedly increased (P < 0.05). CONCLUSION: HUMSC implantation maybe an effective 
      strategy on the treatment of ulceration in diabetic foot rats, and the possible 
      mechanism may involve in BDNF expression.
FAU - Zhou, Ning
AU  - Zhou N
AD  - Institute of Neuroscience, Kunming Medical University, Kunming 650031, China.
FAU - Wang, Qing-Ping
AU  - Wang QP
AD  - Institute of Neurological Disease and Translation Neuroscience Center, West China 
      Hospital, Sichuan University, Chengdu 610041, China.
FAU - Jin, Xing-Fang
AU  - Jin XF
AD  - Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
FAU - Hou, Zong-Liu
AU  - Hou ZL
AD  - Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
FAU - Peng, Bao-Kun
AU  - Peng BK
AD  - Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
FAU - Dan, Qi-Qin
AU  - Dan QQ
AD  - Yan-an Hospital Affiliated to Kunming Medical University, Kunming 650051, China.
FAU - Wang, Tin-Hua
AU  - Wang TH
AD  - Institute of Neuroscience, Kunming Medical University, Kunming 650031, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Diabetes Mellitus, Experimental/complications
MH  - Diabetic Foot/metabolism/*therapy
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Umbilical Cord/*cytology
MH  - Wound Healing/physiology
EDAT- 2014/02/05 06:00
MHDA- 2014/08/30 06:00
CRDT- 2014/02/05 06:00
PHST- 2014/02/05 06:00 [entrez]
PHST- 2014/02/05 06:00 [pubmed]
PHST- 2014/08/30 06:00 [medline]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2013 Nov;44(6):931-4.