PMID- 2449453
OWN - NLM
STAT- MEDLINE
DCOM- 19880407
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 26
IP  - 2
DP  - 1988 Feb
TI  - Simple method for rapid diagnosis of catheter-associated infection by direct 
      acridine orange staining of catheter tips.
PG  - 175-7
AB  - Direct acridine orange (AO) staining was used to detect bacteria adherent to 
      intravascular catheters (IVC). Samples from 710 IVC tips were first cultured on 
      blood agar plates by a semiquantitative technique and then independently colored 
      with AO and screened dry at a magnification of x100 for 3 min. In the absence of 
      fluorescence, they were considered negative. When fluorescence was present, they 
      were further examined for the presence of microorganisms at x1,000 with immersion 
      oil. Of 710 IVC tips, 37 (5.2%) were positive upon culture (greater than or equal 
      to 15 colonies) and 673 were negative (640 were sterile and 33 [4.6%] had 1 to 14 
      colonies). The AO sensitivity was 84%, and the AO specificity was 99%. When 
      restricted to the 212 long IVC, AO sensitivity rose to 94%. AO staining was 
      positive in all cases of catheter-associated bacteremia. The negative predictive 
      value of the preliminary screening at x100 was 99.5%. The direct examination of 
      IVC tips stained by AO appears to be a simple and rapid method for diagnosing 
      IVC-associated infections. In addition, AO staining is easier to perform than 
      Gram staining.
FAU - Zufferey, J
AU  - Zufferey J
AD  - Clinical Bacteriology Laboratory, Centre Hospitalier Universitaire Vaudois, 
      Lausanne, Switzerland.
FAU - Rime, B
AU  - Rime B
FAU - Francioli, P
AU  - Francioli P
FAU - Bille, J
AU  - Bille J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Bacteria/*isolation & purification
MH  - Bacterial Infections/*diagnosis/etiology
MH  - Candida/isolation & purification
MH  - Candidiasis/diagnosis/etiology
MH  - Catheterization, Peripheral/*adverse effects/instrumentation
MH  - Catheters, Indwelling/adverse effects
MH  - Humans
MH  - Microscopy, Fluorescence
MH  - Staining and Labeling
PMC - PMC266246
EDAT- 1988/02/01 00:00
MHDA- 1988/02/01 00:01
PMCR- 1988/02/01
CRDT- 1988/02/01 00:00
PHST- 1988/02/01 00:00 [pubmed]
PHST- 1988/02/01 00:01 [medline]
PHST- 1988/02/01 00:00 [entrez]
PHST- 1988/02/01 00:00 [pmc-release]
AID - 10.1128/jcm.26.2.175-177.1988 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1988 Feb;26(2):175-7. doi: 10.1128/jcm.26.2.175-177.1988.