PMID- 24497370
OWN - NLM
STAT- MEDLINE
DCOM- 20150629
LR  - 20211021
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Print)
IS  - 1549-3296 (Linking)
VI  - 102
IP  - 12
DP  - 2014 Dec
TI  - Poly(ethylene glycol)-containing hydrogels promote the release of primary 
      granules from human blood-derived polymorphonuclear leukocytes.
PG  - 4252-61
LID - 10.1002/jbm.a.35101 [doi]
AB  - Polymorphonuclear leukocytes (PMNs) are recruited to sites of injury and 
      biomaterial implants. Once activated, PMNs can exocytose their granule subsets to 
      recruit monocytes (MCs) and mediate MC/macrophage activation. We investigated the 
      release of myeloperoxidase (MPO), a primary granule marker, and matrix 
      metalloproteinase-9 (MMP-9), a tertiary granule marker, from human blood-derived 
      PMNs cultured on poly(ethylene glycol) (PEG) hydrogels, polydimethylsiloxane 
      (PDMS), tissue culture polystyrene (TCPS) and gelatin-PEG (GP) hydrogels, with 
      and without the presence of the bacterial peptide formyl-Met-Leu-Phe. 
      Supernatants from PMN cultures on PEG-containing hydrogels (i.e., PEG and GP 
      hydrogels) had higher concentrations of MPO than those from PMN cultures on PDMS 
      or TCPS at 2 h. PMNs on all biomaterials released comparable levels of MMP-9 at 2 
      h, indicating that PMNs cultured on PEG-containing hydrogels have different 
      mechanisms of release for primary and tertiary granules. Src family kinases were 
      involved in the release of MPO from PMNs cultured on PEG hydrogels, TCPS and GP 
      hydrogels and in the release of MMP-9 from PMNs cultured on all four 
      biomaterials. The increased release of primary granules from PMNs on 
      PEG-containing hydrogels did not significantly increase MC chemotaxis, indicating 
      that additional co-effectors in the dynamic inflammatory milieu in vivo modulate 
      PMN-mediated MC recruitment.
CI  - (c) 2014 Wiley Periodicals, Inc.
FAU - Cohen, Hannah Caitlin
AU  - Cohen HC
AD  - Pharmaceutical Sciences Division, School of Pharmacy, University of 
      Wisconsin-Madison, 777 Highland Avenue, Madison, Wisconsin, 53705.
FAU - Lieberthal, Tyler Jacob
AU  - Lieberthal TJ
FAU - Kao, W John
AU  - Kao WJ
LA  - eng
GR  - R21 HL115482/HL/NHLBI NIH HHS/United States
GR  - UL1 TR000427/TR/NCATS NIH HHS/United States
GR  - HL115482/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20140213
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Hydrogels)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 3.4.24.35 (MMP9 protein, human)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Chemotaxis
MH  - Culture Media, Conditioned/*chemistry
MH  - Humans
MH  - Hydrogels/*chemistry
MH  - Macrophage Activation/drug effects
MH  - Macrophages/cytology/metabolism
MH  - Matrix Metalloproteinase 9/*chemistry
MH  - Monocytes/cytology/metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/cytology/*metabolism
MH  - Polyethylene Glycols/*chemistry
MH  - Secretory Vesicles/*chemistry
PMC - PMC4121387
MID - NIHMS584594
OTO - NOTNLM
OT  - Neutrophil
OT  - acute inflammation
OT  - degranulation
OT  - matrix metalloproteinase-9
OT  - myeloperoxidase
OT  - poly(ethylene glycol)
EDAT- 2014/02/06 06:00
MHDA- 2015/06/30 06:00
PMCR- 2015/12/01
CRDT- 2014/02/06 06:00
PHST- 2014/01/23 00:00 [received]
PHST- 2014/01/29 00:00 [accepted]
PHST- 2014/02/06 06:00 [entrez]
PHST- 2014/02/06 06:00 [pubmed]
PHST- 2015/06/30 06:00 [medline]
PHST- 2015/12/01 00:00 [pmc-release]
AID - 10.1002/jbm.a.35101 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2014 Dec;102(12):4252-61. doi: 10.1002/jbm.a.35101. Epub 
      2014 Feb 13.