PMID- 24497501
OWN - NLM
STAT- MEDLINE
DCOM- 20141124
LR  - 20221207
IS  - 2045-8827 (Electronic)
IS  - 2045-8827 (Print)
IS  - 2045-8827 (Linking)
VI  - 3
IP  - 2
DP  - 2014 Apr
TI  - Members of the uncultured bacterial candidate division WWE1 are implicated in 
      anaerobic digestion of cellulose.
PG  - 157-67
LID - 10.1002/mbo3.144 [doi]
AB  - Clones of the WWE1 (Waste Water of Evry 1) candidate division were retrieved 
      during the exploration of the bacterial diversity of an anaerobic mesophilic (35 
      +/- 0.5 degrees C) digester. In order to investigate the metabolic function of WWE1 
      members, a 16S rRNA gene -based stable isotope probing (SIP) method was used. 
      Eighty-seven percent of 16S r rRNA gene sequences affiliated to WWE1 candidate 
      division were retrieved in a clone library obtained after polymerase chain 
      reaction (PCR) amplification of enriched DNA fraction from anaerobic municipal 
      solid waste samples incubated with (13) C-cellulose, at the end of the incubation 
      (day 63) using a Pla46F-1390R primer pair. The design of a specific WWE1 probe 
      associated with the fluorescence in situ hybridization (FISH) technique 
      corroborated the abundant representation of WWE1 members in our (13) C-cellulose 
      incubations. Secondary ion mass spectrometry-in situ hybridization (SIMSISH) 
      using an iodine-labeled oligonucleotide probe combined with high-resolution 
      nanometer-scale SIMS (NanoSIMS) observation confirmed the isotopic enrichment of 
      members of WWE1 candidate division. The (13) C apparent isotopic composition of 
      hybridized WWE1 cells reached the value of about 40% early during the cellulose 
      degradation process, suggesting that these bacteria play a role either in an 
      extracellular cellulose hydrolysis process and/or in the uptake fermentation 
      products.
CI  - (c) 2013 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
FAU - Limam, Rim Driss
AU  - Limam RD
AD  - Irstea, UR HBAN, 1 rue Pierre-Gilles de Gennes CS 10030, F-92761, Antony Cedex, 
      France; CEA/Genoscope CNS 2, rue Gaston Cremieux, Evry, 91000, France; University 
      of Evry-Val d'Essonne, Evry, 91057, France; UR04CNSTN01 "Medical and Agricultural 
      Applications of Nuclear Techniques", CNSTN, Sidi Thabet, Ariana, 2020, Tunisia; 
      Faculte des Sciences de Bizerte, Laboratoire de Biochimie et Biologie 
      Moleculaire, 03/UR/0902, Bizerte, Tunisia.
FAU - Chouari, Rakia
AU  - Chouari R
FAU - Mazeas, Laurent
AU  - Mazeas L
FAU - Wu, Ting-Di
AU  - Wu TD
FAU - Li, Tianlun
AU  - Li T
FAU - Grossin-Debattista, Julien
AU  - Grossin-Debattista J
FAU - Guerquin-Kern, Jean-Luc
AU  - Guerquin-Kern JL
FAU - Saidi, Mouldi
AU  - Saidi M
FAU - Landoulsi, Ahmed
AU  - Landoulsi A
FAU - Sghir, Abdelghani
AU  - Sghir A
FAU - Bouchez, Theodore
AU  - Bouchez T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140205
PL  - England
TA  - Microbiologyopen
JT  - MicrobiologyOpen
JID - 101588314
RN  - 0 (Carbon Isotopes)
RN  - 0 (Waste Water)
RN  - 9004-34-6 (Cellulose)
SB  - IM
MH  - Anaerobiosis
MH  - Bacteria/genetics/*isolation & purification/*metabolism
MH  - Biota
MH  - Carbon Isotopes/metabolism
MH  - Cellulose/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Isotope Labeling
MH  - Wastewater/*microbiology
PMC - PMC3996565
OTO - NOTNLM
OT  - Cellulose anaerobic digestion
OT  - FISH
OT  - SIMSISH
OT  - SIP
OT  - WWE1 candidate division
EDAT- 2014/02/06 06:00
MHDA- 2014/12/15 06:00
PMCR- 2014/04/01
CRDT- 2014/02/06 06:00
PHST- 2013/05/31 00:00 [received]
PHST- 2013/10/21 00:00 [revised]
PHST- 2013/10/28 00:00 [accepted]
PHST- 2014/02/06 06:00 [entrez]
PHST- 2014/02/06 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - 10.1002/mbo3.144 [doi]
PST - ppublish
SO  - Microbiologyopen. 2014 Apr;3(2):157-67. doi: 10.1002/mbo3.144. Epub 2014 Feb 5.