PMID- 24498354
OWN - NLM
STAT- MEDLINE
DCOM- 20141203
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 2
DP  - 2014
TI  - PRODH polymorphisms, cortical volumes and thickness in schizophrenia.
PG  - e87686
LID - 10.1371/journal.pone.0087686 [doi]
LID - e87686
AB  - Schizophrenia is a neurodevelopmental disorder with high heritability. Several 
      lines of evidence indicate that the PRODH gene may be related to the disorder. 
      Therefore, our study investigates the effects of 12 polymorphisms of PRODH on 
      schizophrenia and its phenotypes. To further evaluate the roles of the associated 
      variants in the disorder, we have conducted magnetic resonance imaging (MRI) 
      scans to assess cortical volumes and thicknesses. A total of 192 patients were 
      evaluated using the Structured Clinical Interview for DSM-IV (SCID), Positive and 
      Negative Syndrome Scale (PANSS), Calgary Depression Scale, Global Assessment of 
      Functioning (GAF) and Clinical Global Impression (CGI) instruments. The study 
      included 179 controls paired by age and gender. The samples were genotyped using 
      the real-time polymerase chain reaction (PCR), restriction fragment length 
      polymorphism (RFLP)-PCR and Sanger sequencing methods. A sample of 138 patients 
      and 34 healthy controls underwent MRI scans. One polymorphism was associated with 
      schizophrenia (rs2904552), with the G-allele more frequent in patients than in 
      controls. This polymorphism is likely functional, as predicted by PolyPhen and 
      SIFT, but it was not associated with brain morphology in our study. In summary, 
      we report a functional PRODH variant associated with schizophrenia that may have 
      a neurochemical impact, altering brain function, but is not responsible for the 
      cortical reductions found in the disorder.
FAU - Ota, Vanessa K
AU  - Ota VK
AD  - Disciplina de Genetica, Departamento de Morfologia e Genetica, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil ; LiNC - Laboratorio 
      Interdisciplinar de Neurociencias Clinicas, Universidade Federal de Sao Paulo 
      (UNIFESP), Sao Paulo, Sao Paulo, Brazil.
FAU - Bellucco, Fernanda T
AU  - Bellucco FT
AD  - Disciplina de Genetica, Departamento de Morfologia e Genetica, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil.
FAU - Gadelha, Ary
AU  - Gadelha A
AD  - LiNC - Laboratorio Interdisciplinar de Neurociencias Clinicas, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil ; Departamento de 
      Psiquiatria, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, Brazil.
FAU - Santoro, Marcos L
AU  - Santoro ML
AD  - Disciplina de Genetica, Departamento de Morfologia e Genetica, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil ; LiNC - Laboratorio 
      Interdisciplinar de Neurociencias Clinicas, Universidade Federal de Sao Paulo 
      (UNIFESP), Sao Paulo, Sao Paulo, Brazil.
FAU - Noto, Cristiano
AU  - Noto C
AD  - LiNC - Laboratorio Interdisciplinar de Neurociencias Clinicas, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil ; Departamento de 
      Psiquiatria, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, Brazil.
FAU - Christofolini, Denise M
AU  - Christofolini DM
AD  - Disciplina de Genetica e Reproducao Humana, Departamento de Ginecologia e 
      Obstetricia, Faculdade de Medicina do ABC (FMABC), Santo Andre, Sao Paulo, 
      Brazil.
FAU - Assuncao, Idaiane B
AU  - Assuncao IB
AD  - Departamento de Psiquiatria, Universidade Federal de Sao Paulo (UNIFESP), Sao 
      Paulo, Brazil.
FAU - Yamada, Karen M
AU  - Yamada KM
AD  - Disciplina de Genetica, Departamento de Morfologia e Genetica, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil.
FAU - Ribeiro-dos-Santos, Andrea K
AU  - Ribeiro-dos-Santos AK
AD  - Laboratorio de Genetica Humana e Medica, Universidade Federal do Para (UFPA), 
      Belem, Para, Brazil.
FAU - Santos, Sidney
AU  - Santos S
AD  - Laboratorio de Genetica Humana e Medica, Universidade Federal do Para (UFPA), 
      Belem, Para, Brazil.
FAU - Mari, Jair J
AU  - Mari JJ
AD  - Departamento de Psiquiatria, Universidade Federal de Sao Paulo (UNIFESP), Sao 
      Paulo, Brazil.
FAU - Smith, Marilia A C
AU  - Smith MA
AD  - Disciplina de Genetica, Departamento de Morfologia e Genetica, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil.
FAU - Melaragno, Maria I
AU  - Melaragno MI
AD  - Disciplina de Genetica, Departamento de Morfologia e Genetica, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil.
FAU - Bressan, Rodrigo A
AU  - Bressan RA
AD  - LiNC - Laboratorio Interdisciplinar de Neurociencias Clinicas, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil ; Departamento de 
      Psiquiatria, Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, Brazil.
FAU - Sato, Joao R
AU  - Sato JR
AD  - LiNC - Laboratorio Interdisciplinar de Neurociencias Clinicas, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil ; Center of 
      Mathematics, Computation and Cognition, Universidade Federal do ABC, Santo Andre, 
      Brazil.
FAU - Jackowski, Andrea P
AU  - Jackowski AP
AD  - LiNC - Laboratorio Interdisciplinar de Neurociencias Clinicas, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil.
FAU - Belangero, Sintia I
AU  - Belangero SI
AD  - Disciplina de Genetica, Departamento de Morfologia e Genetica, Universidade 
      Federal de Sao Paulo (UNIFESP), Sao Paulo, Sao Paulo, Brazil ; LiNC - Laboratorio 
      Interdisciplinar de Neurociencias Clinicas, Universidade Federal de Sao Paulo 
      (UNIFESP), Sao Paulo, Sao Paulo, Brazil ; Departamento de Psiquiatria, 
      Universidade Federal de Sao Paulo (UNIFESP), Sao Paulo, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140203
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - EC 1.5.3.- (Proline Oxidase)
RN  - EC 1.5.5.2 (PRODH protein, human)
SB  - IM
MH  - Adult
MH  - Cerebral Cortex/*metabolism/pathology
MH  - DNA Mutational Analysis
MH  - Diagnostic and Statistical Manual of Mental Disorders
MH  - Female
MH  - Gene Frequency
MH  - Genetic Predisposition to Disease/*genetics
MH  - Genotype
MH  - Humans
MH  - Linkage Disequilibrium
MH  - Magnetic Resonance Imaging
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Restriction Fragment Length
MH  - *Polymorphism, Single Nucleotide
MH  - Proline Oxidase/*genetics
MH  - Psychiatric Status Rating Scales
MH  - Schizophrenia/diagnosis/enzymology/*genetics
PMC - PMC3912045
COIS- Competing Interests: Dr. Mari served as a symposia speaker from Eli Lilly and 
      Astra Zeneca. Also Dr. Bressan has received honoraria and/or consultations fees 
      from Astra Zeneca, Janssen and Lundbeck; has received research funding from 
      Janssen, Eli Lilly, Lundibeck, Novartis and Roche; and is a shareholder of 
      Biomolecular Technology Ltda. Dr. Gadelha reports having received PhD fellowship 
      from CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico) and 
      served as a speaker from Janssen-Cilag. The other authors have no conflicts of 
      interest to disclose. This does not alter the authors' adherence to all the PLOS 
      ONE policies on sharing data and materials.
EDAT- 2014/02/06 06:00
MHDA- 2014/12/15 06:00
PMCR- 2014/02/03
CRDT- 2014/02/06 06:00
PHST- 2013/05/14 00:00 [received]
PHST- 2014/01/02 00:00 [accepted]
PHST- 2014/02/06 06:00 [entrez]
PHST- 2014/02/06 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
PHST- 2014/02/03 00:00 [pmc-release]
AID - PONE-D-13-19559 [pii]
AID - 10.1371/journal.pone.0087686 [doi]
PST - epublish
SO  - PLoS One. 2014 Feb 3;9(2):e87686. doi: 10.1371/journal.pone.0087686. eCollection 
      2014.