PMID- 24498912
OWN - NLM
STAT- MEDLINE
DCOM- 20150511
LR  - 20161020
IS  - 1439-7609 (Electronic)
IS  - 1439-7595 (Linking)
VI  - 24
IP  - 5
DP  - 2014 Sep
TI  - Identification of citrullinated cellular fibronectin in synovial fluid from 
      patients with rheumatoid arthritis.
PG  - 766-9
LID - 10.3109/14397595.2013.879413 [doi]
AB  - OBJECTIVES: Cellular fibronectin (cFn) has been implicated in the pathogenesis of 
      rheumatoid arthritis (RA), and we previously demonstrated the presence of 
      citrullinated cFn in rheumatoid synovial tissues. The present study aimed to 
      investigate whether citrullinated cFn can be detected in the plasma or synovial 
      fluid of RA patients. METHODS: Twenty-five rheumatoid arthritis synovial fluid 
      (RASF), seven osteoarthritis synovial fluid (OASF) and 12 plasma samples from RA 
      patients were examined. Citrullination of cFn was determined by 
      immunoprecipitation (IP), western blotting and enzyme-linked immunosorbent assay 
      (ELISA), in which peptidyl-citrulline within cFn was detected using a specific 
      anti-cFn monoclonal antibody in combination with anti-modified citrulline 
      antibody after chemical modification. RESULTS: Levels of citrullination 
      associated with cFn, as determined by ELISA, were significantly higher in RASF 
      than in OASF samples. IP and western blotting detected citrullinated cFn in RASF 
      but not in plasma samples from RA patients. Levels of total cFn were elevated in 
      RASF compared with OASF, and 24 out of 25 RASF samples were positive for anti-CCP 
      antibody. However, no correlation was observed between levels of citrullinated 
      cFn and those of total cFn or anti-CCP antibody in RASF. On the other hand, a 
      significant positive correlation was observed between the levels of matrix 
      metalloproteinase-3 (MMP-3) and cFn citrullination in RASF. CONCLUSIONS: 
      Citrullinated cFn appears to be produced within the affected joint and might be 
      involved in the pathogenesis of rheumatoid synovitis.
FAU - Kimura, Eri
AU  - Kimura E
AD  - Department of Rheumatology, Tokyo Medical University Hospital , Tokyo , Japan.
FAU - Kanzaki, Takeyuki
AU  - Kanzaki T
FAU - Tahara, Koichiro
AU  - Tahara K
FAU - Hayashi, Haeru
AU  - Hayashi H
FAU - Hashimoto, Shiori
AU  - Hashimoto S
FAU - Suzuki, Akari
AU  - Suzuki A
FAU - Yamada, Ryo
AU  - Yamada R
FAU - Yamamoto, Kazuhiko
AU  - Yamamoto K
FAU - Sawada, Tetsuji
AU  - Sawada T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140205
PL  - England
TA  - Mod Rheumatol
JT  - Modern rheumatology
JID - 100959226
RN  - 0 (Autoantibodies)
RN  - 0 (Fibronectins)
RN  - 0 (Peptides, Cyclic)
RN  - 0 (cyclic citrullinated peptide)
SB  - IM
MH  - Arthritis, Rheumatoid/immunology/*metabolism
MH  - Autoantibodies/*analysis
MH  - Fibronectins/*metabolism
MH  - Humans
MH  - Osteoarthritis/immunology/metabolism
MH  - Peptides, Cyclic/*immunology
MH  - Synovial Fluid/chemistry/immunology/*metabolism
OTO - NOTNLM
OT  - Cellular fibronectin
OT  - Citrullinated antigen
OT  - Rheumatoid arthritis
OT  - Synovial fluid
EDAT- 2014/02/07 06:00
MHDA- 2015/05/12 06:00
CRDT- 2014/02/07 06:00
PHST- 2014/02/07 06:00 [entrez]
PHST- 2014/02/07 06:00 [pubmed]
PHST- 2015/05/12 06:00 [medline]
AID - 10.3109/14397595.2013.879413 [doi]
PST - ppublish
SO  - Mod Rheumatol. 2014 Sep;24(5):766-9. doi: 10.3109/14397595.2013.879413. Epub 2014 
      Feb 5.