PMID- 24499049
OWN - NLM
STAT- MEDLINE
DCOM- 20141031
LR  - 20140310
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 15
IP  - 3
DP  - 2014 Mar 10
TI  - Potential of Ni(II)-NTA-modified poly(ethylene imine) glycopolymers as carrier 
      system for future dendritic cell-based immunotherapy.
PG  - 957-67
LID - 10.1021/bm401845b [doi]
AB  - Dendritic cells (DCs) play a crucial role in the development of cell-mediated 
      immunotherapy due to their ability to induce and maintain strong immune 
      responses. In our study, we evaluated a biocompatible Ni(II)-NTA-modified 
      poly(ethylene imine) dendritic glycopolymer (Ni(II)-NTA-DG) as new carrier system 
      to increase the antigen uptake into iDCs for future DC-based immunotherapy. 
      Ni(II)-NTA-DG led to an increase in His6-Gp160 uptake in monocytes and iDCs, 
      where His6-Gp160 is localized in the early endosomal and lysosomal compartments. 
      Ni(II)-NTA-DG and the formed polyplexes induced an activation of iDCs, showing an 
      increasing expression of costimulatory molecules CD86, CD80, and proinflammatory 
      cytokines IL-6 and IL-8. Beside no influencing effect of Ni(II)-NTA-DG and 
      polyplexes on the maturation of antigen-bearing DCs, the mature peptide bearing 
      DCs remained their ability to migrate along a gradient of CCR7 ligands. Thus, 
      Ni(II)-NTA-DG with advancing biological properties is a promising carrier system 
      for the future application in DC-based immunotherapy.
FAU - Hauptmann, N
AU  - Hauptmann N
AD  - Leibniz-Institut fur Polymerforschung Dresden e.V. , Hohe Strasse 6, D-01069 
      Dresden, Germany.
FAU - Pion, M
AU  - Pion M
FAU - Wehner, R
AU  - Wehner R
FAU - Munoz-Fernandez, M-A
AU  - Munoz-Fernandez MA
FAU - Schmitz, M
AU  - Schmitz M
FAU - Voit, B
AU  - Voit B
FAU - Appelhans, D
AU  - Appelhans D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140213
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Imines)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Polyethylenes)
RN  - 0 (poly(ethylene imine))
SB  - IM
MH  - Cell Differentiation/genetics/immunology
MH  - Dendritic Cells/chemistry/*cytology/immunology
MH  - Flow Cytometry
MH  - Humans
MH  - Imines/*chemistry/immunology
MH  - *Immunotherapy
MH  - Interleukin-6/chemistry/metabolism
MH  - Interleukin-8/chemistry/metabolism
MH  - Lipopolysaccharides/metabolism
MH  - Monocytes/cytology
MH  - Polyethylenes/*chemistry
EDAT- 2014/02/07 06:00
MHDA- 2014/11/02 06:00
CRDT- 2014/02/07 06:00
PHST- 2014/02/07 06:00 [entrez]
PHST- 2014/02/07 06:00 [pubmed]
PHST- 2014/11/02 06:00 [medline]
AID - 10.1021/bm401845b [doi]
PST - ppublish
SO  - Biomacromolecules. 2014 Mar 10;15(3):957-67. doi: 10.1021/bm401845b. Epub 2014 
      Feb 13.