PMID- 24499596
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20140227
LR  - 20220408
IS  - 1755-8166 (Print)
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 7
IP  - 1
DP  - 2014 Feb 5
TI  - Establishment of a molecular cytogenetic analysis for native tumor tissue of 
      meningiomas-suitable for clinical application.
PG  - 12
LID - 10.1186/1755-8166-7-12 [doi]
AB  - BACKGROUND: Meningiomas are mostly benign tumors which arise from the meninges. 
      They are among the cytogenetically best-studied solid tumors, mostly displaying a 
      normal karyotype or, as a typical primary aberration, monosomy of chromosome 22. 
      Further secondary chromosomal aberrations, especially the deletion of chromosome 
      1p, are correlated with increasing biological aggressiveness up to malignancy. 
      These data are derived from the cytogenetical characterization of 661 
      meningiomas, from which the genetic progression score (GPS) has been developed. 
      Due to the high expenditure of time and the expert knowledge for the 
      cytogenetical characterization, the aim of this work was to establish an equally 
      reliable yet more rapid clinical diagnosis based on fluorescence in situ 
      hybridization (FISH) on meningiomas. Thus a comparison between the native tumor 
      tissue and the primary culture of the same tumor was done in order to determine 
      the most efficient method for a molecular cytogenetic characterization. The 
      diagnostic procedure has to deliver fast and robust results, since they must 
      enable the attending physician to plan the appropriate follow-up regimens for the 
      patients. All in all, preparations of native tumor tissue as well as preparations 
      of cell culture of 22 meningiomas were tested with FISH for aberrations 
      concerning the prognostically relevant chromosome regions 1p and 9p, and the 
      chromosomes 10, 14, 18 and 22 in comparison with the particular karyotypes 
      revealed by conventional karyotyping using G-banding. RESULTS: The FISH 
      examinations between native and cultured cells showed an accordance of 93.4%. The 
      comparison of FISH data and karyotyping presented accordance to the greatest 
      possible extent concerning the chromosomes 14, 18 and 22, but to detect the 
      progression associated losses of 1p and 9p FISH is the most sensitive method. 
      CONCLUSIONS: The raised data reveal that both methods can be used for a 
      significant analysis of chromosome aberrations on meningiomas. As a result of 
      that the complex primary culture could also be avoided. Therefore a clinical 
      diagnosis based on FISH on meningiomas is at hand for the assignment of patients 
      to a suitable follow-up regimen.
FAU - Lerner, Cornelia
AU  - Lerner C
AD  - Department of Otolaryngology, Saarland University, Homburg/Saar, Germany.
FAU - Ketter, Ralf
AU  - Ketter R
AD  - Department of Neurosurgery, Saarland University, Homburg/Saar D-66421, Germany.
FAU - Linsler, Stefan
AU  - Linsler S
AD  - Department of Neurosurgery, Saarland University, Homburg/Saar D-66421, Germany.
FAU - Henn, Wolfram
AU  - Henn W
AD  - Institute of Human Genetics, Saarland University, Homburg/Saar, Germany.
FAU - Oertel, Joachim
AU  - Oertel J
AD  - Department of Neurosurgery, Saarland University, Homburg/Saar D-66421, Germany.
FAU - Urbschat, Steffi
AU  - Urbschat S
AD  - Department of Neurosurgery, Saarland University, Homburg/Saar D-66421, Germany.
LA  - eng
PT  - Journal Article
DEP - 20140205
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC3937053
EDAT- 2014/02/07 06:00
MHDA- 2014/02/07 06:01
PMCR- 2014/02/05
CRDT- 2014/02/07 06:00
PHST- 2013/12/16 00:00 [received]
PHST- 2014/01/15 00:00 [accepted]
PHST- 2014/02/07 06:00 [entrez]
PHST- 2014/02/07 06:00 [pubmed]
PHST- 2014/02/07 06:01 [medline]
PHST- 2014/02/05 00:00 [pmc-release]
AID - 1755-8166-7-12 [pii]
AID - 10.1186/1755-8166-7-12 [doi]
PST - epublish
SO  - Mol Cytogenet. 2014 Feb 5;7(1):12. doi: 10.1186/1755-8166-7-12.