PMID- 24500399
OWN - NLM
STAT- MEDLINE
DCOM- 20141205
LR  - 20211021
IS  - 1476-5470 (Electronic)
IS  - 1466-4879 (Linking)
VI  - 15
IP  - 3
DP  - 2014 Apr-May
TI  - High-allelic variability in HLA-C mRNA expression: association with HLA-extended 
      haplotypes.
PG  - 176-81
LID - 10.1038/gene.2014.1 [doi]
AB  - Human leukocyte antigen (HLA)-C is a clinically relevant transplantation antigen 
      in unrelated hematopoietic stem cell and cord blood transplantation. Furthermore, 
      HLA-C antigens, as ligands for killer immunoglobulin-like receptors expressed on 
      natural killer cells, have a central role in HIV control. Several studies have 
      reported significant correlations between HLA-C mRNA and cell surface expression 
      with polymorphisms in the 5'- and 3'-regions of the HLA-C locus. We determined 
      HLA-C mRNA in blood donors by using locus as well as allele-specific 
      real-time-PCR and focused the analysis on HLA-extended haplotypes. High 
      inter-individual variability of mRNA expression was disclosed. A lower 
      inter-individual variability for C*07:01 but a higher variability for C*06:02, 
      C*04:01 and C*03:04 alleles were detected. The previously reported associations 
      between HLA-C cell surface expression and -32 kb/-35 kb single nucleotide 
      polymorphisms were not confirmed. Related and unrelated individuals sharing the 
      same two A-B-C-DRB1 or B-C haplotypes show strikingly similar levels of HLA-C 
      mRNA expression in each of the different haplotypic combinations tested. 
      Altogether, our results suggest that HLA-C expression levels best correlate with 
      the extended HLA haplotype rather than with the allotype or with polymorphisms in 
      the 5'-region of the HLA-C locus.
FAU - Bettens, F
AU  - Bettens F
AD  - National Reference Laboratory for Histocompatibility, Transplantation Immunology 
      Unit, Department of Genetics and Laboratory Medicine, University Hospital, 
      Geneva, Switzerland.
FAU - Brunet, L
AU  - Brunet L
AD  - National Reference Laboratory for Histocompatibility, Transplantation Immunology 
      Unit, Department of Genetics and Laboratory Medicine, University Hospital, 
      Geneva, Switzerland.
FAU - Tiercy, J-M
AU  - Tiercy JM
AD  - National Reference Laboratory for Histocompatibility, Transplantation Immunology 
      Unit, Department of Genetics and Laboratory Medicine, University Hospital, 
      Geneva, Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140206
PL  - England
TA  - Genes Immun
JT  - Genes and immunity
JID - 100953417
RN  - 0 (HLA-C Antigens)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - *Alleles
MH  - *Gene Expression
MH  - HLA-C Antigens/*genetics
MH  - *Haplotypes
MH  - Humans
MH  - Polymorphism, Single Nucleotide
MH  - *RNA, Messenger
EDAT- 2014/02/07 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/02/07 06:00
PHST- 2013/10/08 00:00 [received]
PHST- 2013/12/06 00:00 [revised]
PHST- 2013/12/13 00:00 [accepted]
PHST- 2014/02/07 06:00 [entrez]
PHST- 2014/02/07 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - gene20141 [pii]
AID - 10.1038/gene.2014.1 [doi]
PST - ppublish
SO  - Genes Immun. 2014 Apr-May;15(3):176-81. doi: 10.1038/gene.2014.1. Epub 2014 Feb 
      6.