PMID- 24500949
OWN - NLM
STAT- MEDLINE
DCOM- 20141104
LR  - 20211021
IS  - 1095-8290 (Electronic)
IS  - 0305-7364 (Print)
IS  - 0305-7364 (Linking)
VI  - 113
IP  - 4
DP  - 2014 Mar
TI  - Combining FISH and model-based predictions to understand chromosome evolution in 
      Typhonium (Araceae).
PG  - 669-80
LID - 10.1093/aob/mct302 [doi]
AB  - BACKGROUND AND AIMS: Since the advent of molecular phylogenetics, numerous 
      attempts have been made to infer the evolutionary trajectories of chromosome 
      numbers on DNA phylogenies. Ideally, such inferences should be evaluated against 
      cytogenetic data. Towards this goal, we carried out phylogenetic modelling of 
      chromosome number change and fluorescence in situ hybridization (FISH) in a 
      medium sized genus of Araceae to elucidate if data from chromosomal markers would 
      support maximum likelihood-inferred changes in chromosome numbers among close 
      relatives. Typhonium, the focal genus, includes species with 2n = 65 and 2n = 8, 
      the lowest known count in the family. METHODS: A phylogeny from nuclear and 
      plastid sequences (96 taxa, 4252 nucleotides) and counts for all included species 
      (15 of them first reported here) were used to model chromosome number evolution, 
      assuming discrete events, such as polyploidization and descending or ascending 
      dysploidy, occurring at different rates. FISH with three probes (5S rDNA, 45S 
      rDNA and Arabidopsis-like telomeres) was performed on ten species with 2n = 8 to 
      2n = 24. KEY RESULTS: The best-fitting models assume numerous past chromosome 
      number reductions. Of the species analysed with FISH, the two with the lowest 
      chromosome numbers contained interstitial telomeric signals (Its), which together 
      with the phylogeny and modelling indicates decreasing dysploidy as an explanation 
      for the low numbers. A model-inferred polyploidization in another species is 
      matched by an increase in rDNA sites. CONCLUSIONS: The combination of a densely 
      sampled phylogeny, ancestral state modelling and FISH revealed that the species 
      with n = 4 is highly derived, with the FISH data pointing to a Robertsonian 
      fusion-like chromosome rearrangement in the ancestor of this species.
FAU - Sousa, Aretuza
AU  - Sousa A
AD  - Department of Biology, University of Munich (LMU), D-80638 Munich, Germany.
FAU - Cusimano, Natalie
AU  - Cusimano N
FAU - Renner, Susanne S
AU  - Renner SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140204
PL  - England
TA  - Ann Bot
JT  - Annals of botany
JID - 0372347
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Aneuploidy
MH  - Araceae/cytology/*genetics
MH  - Base Sequence
MH  - Chromosomes, Plant/*genetics
MH  - Cytogenetic Analysis
MH  - DNA, Plant/genetics
MH  - DNA, Ribosomal/genetics
MH  - Evolution, Molecular
MH  - Genetic Markers/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Likelihood Functions
MH  - Phylogeny
MH  - Plastids/genetics
MH  - Species Specificity
MH  - Telomere
PMC - PMC3936593
OTO - NOTNLM
OT  - 45S rDNA
OT  - 5S rDNA
OT  - Ancestral trait reconstruction
OT  - Araceae
OT  - Bayesian inference
OT  - FISH
OT  - Typhonium
OT  - aneuploid chromosome numbers
OT  - chromosome evolution
OT  - maximum likelihood inference
OT  - telomeres
EDAT- 2014/02/07 06:00
MHDA- 2014/11/05 06:00
PMCR- 2015/03/01
CRDT- 2014/02/07 06:00
PHST- 2014/02/07 06:00 [entrez]
PHST- 2014/02/07 06:00 [pubmed]
PHST- 2014/11/05 06:00 [medline]
PHST- 2015/03/01 00:00 [pmc-release]
AID - mct302 [pii]
AID - 10.1093/aob/mct302 [doi]
PST - ppublish
SO  - Ann Bot. 2014 Mar;113(4):669-80. doi: 10.1093/aob/mct302. Epub 2014 Feb 4.