PMID- 24500988 OWN - NLM STAT- MEDLINE DCOM- 20150110 LR - 20181202 IS - 1095-8355 (Electronic) IS - 1065-6995 (Linking) VI - 38 IP - 6 DP - 2014 Jun TI - Rat cortex and hippocampus-derived soluble factors for the induction of adipose-derived mesenchymal stem cells into neuron-like cells. PG - 768-76 LID - 10.1002/cbin.10256 [doi] AB - To simulate brain microenvironment, adipose-derived mesenchymal stem cells (AMSC) were induced to differentiate to neuronal-like cells in rat cortex and hippocampus medium (Cox + Hip). First, isolated AMSC were characterized by flow cytometer and the capacity of adipogenesis and osteogenesis. After induction in rat cortex and hippocampus conditioned medium, the cell morphological change was examined and neural marker proteins (beta-capital SHA, Cyrillic-Tubulin, NSE, Nissl body) expression was detected by immunofluorescence staining. A variety of synaptic marker proteins, including GAP43, SHANK2, SHANK3 and Bassoon body, were detected. ELISA was used to measure brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) secretion at different time-points. AMSCs positively expressed CD13, CD44 and CD90 and could differentiate into osteoblasts or adipocytes. After induction in Cox + Hip medium for 14 days, cells had a typical neuronal perikaryal appearance, which was suggestive of neuronal differentiation. After 14 days of Cox + Hip treatment, the percentage of cells expressing beta-Ⅲ-Tubulin, NSE and Nissl was 53.9 +/- 0.8%, 51.3 +/- 1.7% and 16.4 +/- 2.1%, respectively. Expression of GAP43, SHANK2, SHANK3 and Bassoon body was detected, indicating synapse formation after treatment in Cox + Hip medium. Differentiated AMSCs secreted neurotrophic factors NGF and BDNF. Thus rat cortex and hippocampus-derived soluble factors can induce AMSCs to a neuronal-like phenotype, suggesting that AMSCs have a dual role in supplementing newborn neurons and secreting neurotrophic factors, and therefore could be help as a potential treatment for nervous system diseases. CI - (c) 2014 International Federation for Cell Biology. FAU - Han, Chao AU - Han C AD - Regenerative Medicine Centre, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, P.R. China; Institute of Integrative Medicine, Dalian Medical University, Dalian, 116011, P.R. China. FAU - Song, Lin AU - Song L FAU - Liu, Yang AU - Liu Y FAU - Zou, Wei AU - Zou W FAU - Jiang, Chen AU - Jiang C FAU - Liu, Jing AU - Liu J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20140220 PL - England TA - Cell Biol Int JT - Cell biology international JID - 9307129 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Bsn protein, rat) RN - 0 (GAP-43 Protein) RN - 0 (Hyaluronan Receptors) RN - 0 (Nerve Tissue Proteins) RN - 0 (Shank2 protein, rat) RN - 0 (Shank3 protein, rat) RN - 0 (Thy-1 Antigens) RN - 0 (Tubb3 protein, rat) RN - 0 (Tubulin) RN - 9061-61-4 (Nerve Growth Factor) RN - EC 3.4.11.2 (CD13 Antigens) SB - IM MH - Adipocytes/*cytology MH - Adipose Tissue/*cytology/metabolism MH - Adult MH - Animals MH - Brain-Derived Neurotrophic Factor/metabolism MH - CD13 Antigens/biosynthesis MH - Cerebellar Cortex/cytology/metabolism MH - GAP-43 Protein/biosynthesis MH - Hippocampus/*cytology MH - Humans MH - Hyaluronan Receptors/biosynthesis MH - Mesenchymal Stem Cells/*metabolism MH - Middle Aged MH - Nerve Growth Factor/metabolism MH - Nerve Tissue Proteins/biosynthesis MH - Neurogenesis/*physiology MH - Nissl Bodies/metabolism MH - Osteoblasts/cytology MH - Rats MH - Thy-1 Antigens/biosynthesis MH - Tubulin/biosynthesis MH - Young Adult OTO - NOTNLM OT - adipose-derived mesenchymal stem cells (AMSCs) OT - cortex and hippocampus- conditioned medium OT - neuronal differentiation EDAT- 2014/02/07 06:00 MHDA- 2015/01/13 06:00 CRDT- 2014/02/07 06:00 PHST- 2013/08/09 00:00 [received] PHST- 2014/01/27 00:00 [accepted] PHST- 2014/02/07 06:00 [entrez] PHST- 2014/02/07 06:00 [pubmed] PHST- 2015/01/13 06:00 [medline] AID - 10.1002/cbin.10256 [doi] PST - ppublish SO - Cell Biol Int. 2014 Jun;38(6):768-76. doi: 10.1002/cbin.10256. Epub 2014 Feb 20.