PMID- 24503075
OWN - NLM
STAT- MEDLINE
DCOM- 20140502
LR  - 20140207
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 450-451
DP  - 2014 Feb
TI  - Computer-aided codon-pairs deoptimization of the major envelope GP5 gene 
      attenuates porcine reproductive and respiratory syndrome virus.
PG  - 132-9
LID - S0042-6822(13)00672-7 [pii]
LID - 10.1016/j.virol.2013.12.009 [doi]
AB  - Synthetic attenuated virus engineering (SAVE) is an emerging technology that 
      enables rapid attenuation of viruses. In this study, by using SAVE we 
      demonstrated rapid attenuation of an arterivirus, porcine reproductive and 
      respiratory syndrome virus (PRRSV). The major envelope GP5 gene of PRRSV was 
      codon-pair deoptimized aided by a computer algorithm. The codon-pair deoptimized 
      virus, designated as SAVE5 with a deoptimized GP5 gene, was successfully rescued 
      in vitro. The SAVE5 virus replicated at a lower level in vitro with a significant 
      decrease of GP5 protein expression compared to the wild-type PRRSV VR2385 virus. 
      Pigs experimentally infected with the SAVE5 virus had significantly lower viremia 
      level up to 14 days post-infection as well as significantly reduced gross and 
      histological lung lesions when compared to wild-type PRRSV VR2385 virus-infected 
      pigs, indicating the attenuation of the SAVE5 virus. This study proved the 
      feasibility of rapidly attenuating PRRSV by SAVE.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Ni, Yan-Yan
AU  - Ni YY
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA, USA.
FAU - Zhao, Zhao
AU  - Zhao Z
AD  - Department of Computer Science, Virginia Polytechnic Institute and State 
      University, Blacksburg, VA, USA.
FAU - Opriessnig, Tanja
AU  - Opriessnig T
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Subramaniam, Sakthivel
AU  - Subramaniam S
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA, USA.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA, USA; College of Veterinary 
      Medicine, China Agricultural University, Beijing, People's Republic of China.
FAU - Cao, Dianjun
AU  - Cao D
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA, USA.
FAU - Cao, Qian
AU  - Cao Q
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA, USA.
FAU - Yang, Hanchun
AU  - Yang H
AD  - College of Veterinary Medicine, China Agricultural University, Beijing, People's 
      Republic of China.
FAU - Meng, Xiang-Jin
AU  - Meng XJ
AD  - Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA, USA. Electronic address: 
      xjmeng@vt.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20131231
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Codon)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Codon
MH  - Computer-Aided Design
MH  - *Genetic Engineering
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/physiology
MH  - Swine
MH  - Viral Envelope Proteins/*genetics/metabolism
MH  - Virus Replication
OTO - NOTNLM
OT  - Attenuation
OT  - GP5 gene
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Synthetic attenuated virus engineering (SAVE)
EDAT- 2014/02/08 06:00
MHDA- 2014/05/03 06:00
CRDT- 2014/02/08 06:00
PHST- 2013/08/17 00:00 [received]
PHST- 2013/09/11 00:00 [revised]
PHST- 2013/12/10 00:00 [accepted]
PHST- 2014/02/08 06:00 [entrez]
PHST- 2014/02/08 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
AID - S0042-6822(13)00672-7 [pii]
AID - 10.1016/j.virol.2013.12.009 [doi]
PST - ppublish
SO  - Virology. 2014 Feb;450-451:132-9. doi: 10.1016/j.virol.2013.12.009. Epub 2013 Dec 
      31.