PMID- 24503088
OWN - NLM
STAT- MEDLINE
DCOM- 20140502
LR  - 20140207
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 450-451
DP  - 2014 Feb
TI  - Generation and characterization of mouse monoclonal antibodies against NS4B 
      protein of dengue virus.
PG  - 250-7
LID - S0042-6822(13)00688-0 [pii]
LID - 10.1016/j.virol.2013.12.025 [doi]
AB  - Dengue virus (DENV) non-structural protein 4B (NS4B) has been demonstrated to be 
      an attractive antiviral target. Due to its nature as an integral membrane 
      protein, NS4B remains poorly characterized. In this study, we generated and 
      characterized two monoclonal antibodies (mAb) that selectively bind to DENV NS4B 
      protein. One mAb, 10-3-7, is specific for DENV-2 NS4B, and its epitope was mapped 
      to residues 5-15 of NS4B. The other mAb, 44-4-7, cross-reacts with all the four 
      serotypes of DENV NS4B, and its epitope was mapped to residues 141-147 of NS4B. 
      Using the mAbs, we probed the intracellular orientation of the epitopes of NS4B 
      by an epitope accessibility assay. The results showed that the N-terminus of NS4B 
      is located in the ER lumen, whereas amino acids 130-148 of NS4B are located in 
      the cytosol. The study demonstrates that the two anti-NS4B mAbs will be useful 
      for future structural and functional analyses of DENV NS4B.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Xie, Xuping
AU  - Xie X
AD  - Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01, Chromos, 
      Singapore 138670, Singapore.
FAU - Zou, Jing
AU  - Zou J
AD  - Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01, Chromos, 
      Singapore 138670, Singapore.
FAU - Wang, Qing-Yin
AU  - Wang QY
AD  - Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01, Chromos, 
      Singapore 138670, Singapore. Electronic address: qing_yin.wang@novartis.com.
FAU - Noble, Christian G
AU  - Noble CG
AD  - Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01, Chromos, 
      Singapore 138670, Singapore.
FAU - Lescar, Julien
AU  - Lescar J
AD  - School of Biological Sciences, Nanyang Technological University, Singapore.
FAU - Shi, Pei-Yong
AU  - Shi PY
AD  - Novartis Institute for Tropical Diseases, 10 Biopolis Road, 05-01, Chromos, 
      Singapore 138670, Singapore. Electronic address: pei_yong.shi@novartis.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140109
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibodies, Viral/*immunology
MH  - Cross Reactions
MH  - Dengue/*immunology/virology
MH  - Dengue Virus/chemistry/genetics/*immunology
MH  - Epitope Mapping
MH  - Female
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Viral Nonstructural Proteins/chemistry/genetics/*immunology
OTO - NOTNLM
OT  - Dengue virus NS4B protein
OT  - Flavivirus replication
OT  - Membrane protein
EDAT- 2014/02/08 06:00
MHDA- 2014/05/03 06:00
CRDT- 2014/02/08 06:00
PHST- 2013/09/23 00:00 [received]
PHST- 2013/10/13 00:00 [revised]
PHST- 2013/12/17 00:00 [accepted]
PHST- 2014/02/08 06:00 [entrez]
PHST- 2014/02/08 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
AID - S0042-6822(13)00688-0 [pii]
AID - 10.1016/j.virol.2013.12.025 [doi]
PST - ppublish
SO  - Virology. 2014 Feb;450-451:250-7. doi: 10.1016/j.virol.2013.12.025. Epub 2014 Jan 
      9.