PMID- 24503757
OWN - NLM
STAT- MEDLINE
DCOM- 20140527
LR  - 20151119
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Linking)
VI  - 38
IP  - 5
DP  - 2014 May
TI  - p16 FISH deletion in surface epithelial mesothelial proliferations is predictive 
      of underlying invasive mesothelioma.
PG  - 681-8
LID - 10.1097/PAS.0000000000000176 [doi]
AB  - An atypical mesothelial proliferation along the pleural or peritoneal surface 
      without evidence of invasive tumor poses a diagnostic challenge. Homozygous 
      deletion of p16 (CDKN2A) by fluorescence in situ hybridization (FISH) has been 
      shown to be a good marker of malignancy in mesothelial proliferations, but 
      correlations of p16 status between atypical surface proliferations and underlying 
      mesothelioma have not been described. We used p16 FISH to investigate 11 pleural 
      and 7 peritoneal mesotheliomas that had both an invasive component and a separate 
      surface mesothelial proliferation. In 5/11 pleural samples and 1/7 peritoneal 
      samples, the invasive mesotheliomas showed homozygous deletion of p16 (all cases 
      in excess of 90% of cells deleted); the surface proliferation in all 6 cases with 
      deletion in the invasive tumor was also p16 deleted. Conversely, the 12 tumors 
      that did not show p16 deletion in the invasive compartment also did not have 
      deletion in the surface component. We conclude that (1) surface mesothelial 
      proliferations near invasive mesotheliomas show the same pattern of p16 by FISH 
      as the underlying tumor and may represent in situ disease or growth of the 
      underlying mesothelioma along the serosal surface; (2) p16 deletion in 
      mesothelial surface proliferations is strongly associated with p16 deletion in 
      underlying mesotheliomas, and biopsies consisting of pure surface mesothelial 
      proliferations that are p16 deleted allow a diagnosis of mesothelioma without an 
      additional biopsy if there is clinical (thoracosopic/laparoscopic) or radiologic 
      evidence of diffuse pleural or peritoneal tumor; (3) however, the absence of p16 
      deletion in surface proliferations does not rule out underlying invasive 
      mesothelioma.
FAU - Hwang, Harry
AU  - Hwang H
AD  - *PhenoPath Laboratories, Seattle, WA daggerDepartment of Pathology, Vancouver General 
      Hospital and University of British Columbia, Vancouver, BC, Canada.
FAU - Tse, Christopher
AU  - Tse C
FAU - Rodriguez, Stephanie
AU  - Rodriguez S
FAU - Gown, Allen
AU  - Gown A
FAU - Churg, Andrew
AU  - Churg A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
SB  - IM
MH  - Biomarkers, Tumor/*genetics
MH  - Cell Proliferation
MH  - Cyclin-Dependent Kinase Inhibitor p16/genetics
MH  - Gene Deletion
MH  - *Genes, p16
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mesothelioma/*diagnosis/*genetics
EDAT- 2014/02/08 06:00
MHDA- 2014/05/28 06:00
CRDT- 2014/02/08 06:00
PHST- 2014/02/08 06:00 [entrez]
PHST- 2014/02/08 06:00 [pubmed]
PHST- 2014/05/28 06:00 [medline]
AID - 10.1097/PAS.0000000000000176 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2014 May;38(5):681-8. doi: 10.1097/PAS.0000000000000176.