PMID- 24504073
OWN - NLM
STAT- MEDLINE
DCOM- 20150226
LR  - 20230210
IS  - 1530-0285 (Electronic)
IS  - 0893-3952 (Linking)
VI  - 27
IP  - 9
DP  - 2014 Sep
TI  - A novel approach for HLA-A typing in formalin-fixed paraffin-embedded-derived 
      DNA.
PG  - 1296-305
LID - 10.1038/modpathol.2013.210 [doi]
AB  - The aim of this study was to establish a novel approach for human leukocyte 
      antigen (HLA)-typing from formalin-fixed paraffin-embedded-derived DNA. HLAs can 
      be a prognostic factor in cancer and have an extensive polymorphism. This 
      polymorphism is predominantly restricted to exons, which encode the 
      peptide-binding domain of the protein. Formalin-fixed paraffin-embedded material 
      is routinely collected in the clinic and therefore a great source of DNA for 
      genetic analyses. However, its low quality due to fragmentation and nucleotide 
      changes has often created obstacles in designing genetic assays. In this study, 
      we amplified the most polymorphic exons of the HLA-A gene, exons 2, 3, and 4, in 
      16 formalin-fixed paraffin-embedded samples >10 years old. These tissue samples 
      belonged to patients already HLA-typed by peripheral blood samples at the routine 
      laboratory. Acquired amplification products were used for sequencing, which 
      provided enough information to establish an HLA allele. The same method was 
      applied to DNA extracted from peripheral blood from a healthy volunteer with 
      known HLA type. Of the samples, 14/16 (88%) were successfully typed, in one 
      sample only one of the alleles could be determined, and in one sample no allele 
      could be determined. The amplification of the most polymorphic exons of HLA-A was 
      a successful alternative when DNA quality prevented positive results with 
      previously described methods. The method is usable when an HLA type is needed but 
      the patients are deceased and/or no whole blood samples can be collected. It has 
      thus potential to be used in several fields such as the clinic, research, and 
      forensic science.
FAU - Villabona, Lisa
AU  - Villabona L
AD  - Department of Oncology-Pathology, Karolinska Institute, Radiumhemmet, P301057 
      Karolinska University Hospital, Stockholm, Sweden.
FAU - Leon Rodriguez, Daniel A
AU  - Leon Rodriguez DA
AD  - 1] Department of Oncology-Pathology, Karolinska Institute, Radiumhemmet, P301057 
      Karolinska University Hospital, Stockholm, Sweden [2] Escuela de Medicina y 
      Ciencias de la Salud, Universidad del Rosario, Bogota, Colombia.
FAU - Andersson, Emilia K
AU  - Andersson EK
AD  - Department of Oncology-Pathology, Karolinska Institute, Radiumhemmet, P301057 
      Karolinska University Hospital, Stockholm, Sweden.
FAU - Seliger, Barbara
AU  - Seliger B
AD  - Institute for Medical Immunology, Halle, Germany.
FAU - Dalianis, Tina
AU  - Dalianis T
AD  - Department of Oncology-Pathology, Karolinska Institute, Radiumhemmet, P301057 
      Karolinska University Hospital, Stockholm, Sweden.
FAU - Masucci, Giuseppe V
AU  - Masucci GV
AD  - Department of Oncology-Pathology, Karolinska Institute, Radiumhemmet, P301057 
      Karolinska University Hospital, Stockholm, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20140207
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (DNA Primers)
RN  - 0 (Fixatives)
RN  - 0 (HLA-A Antigens)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alleles
MH  - Base Sequence
MH  - DNA/*analysis
MH  - DNA Primers
MH  - Exons
MH  - Female
MH  - Fixatives
MH  - Formaldehyde
MH  - Gene Amplification
MH  - HLA-A Antigens/*genetics
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Molecular Sequence Data
MH  - Ovarian Neoplasms/*genetics
MH  - Paraffin Embedding
MH  - Polymerase Chain Reaction/methods
EDAT- 2014/02/08 06:00
MHDA- 2015/02/27 06:00
CRDT- 2014/02/08 06:00
PHST- 2013/05/27 00:00 [received]
PHST- 2013/10/01 00:00 [revised]
PHST- 2013/10/06 00:00 [accepted]
PHST- 2014/02/08 06:00 [entrez]
PHST- 2014/02/08 06:00 [pubmed]
PHST- 2015/02/27 06:00 [medline]
AID - S0893-3952(22)03575-X [pii]
AID - 10.1038/modpathol.2013.210 [doi]
PST - ppublish
SO  - Mod Pathol. 2014 Sep;27(9):1296-305. doi: 10.1038/modpathol.2013.210. Epub 2014 
      Feb 7.