PMID- 24505254
OWN - NLM
STAT- MEDLINE
DCOM- 20141108
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 9
IP  - 2
DP  - 2014
TI  - Comparative evaluation of differentiation potential of menstrual blood- versus 
      bone marrow-derived stem cells into hepatocyte-like cells.
PG  - e86075
LID - 10.1371/journal.pone.0086075 [doi]
LID - e86075
AB  - Menstrual blood has been introduced as an easily accessible and refreshing stem 
      cell source with no ethical consideration. Although recent works have shown that 
      menstrual blood stem cells (MenSCs) possess multi lineage differentiation 
      capacity, their efficiency of hepatic differentiation in comparison to other stem 
      cell resources has not been addressed so far. The aim of this study was to 
      investigate hepatic differentiation capacity of MenSCs compared to bone 
      marrow-derived stem cells (BMSCs) under protocols developed by different 
      concentrations of hepatocyte growth factor (HGF) and oncostatin M (OSM) in 
      combination with other components in serum supplemented or serum-free culture 
      media. Such comparison was made after assessment of immunophenotye, 
      trans-differentiation potential, immunogenicity and tumorigeicity of these cell 
      types. The differential expression of mature hepatocyte markers such as albumin 
      (ALB), cytokeratin 18 (CK-18), tyrosine aminotransferase and cholesterol 7 
      alpha-hydroxylase activities (CYP7A1) at both mRNA and protein levels in 
      differentiating MenSCs was significantly higher in upper concentration of HGF and 
      OSM (P1) compared to lower concentration of these factors (P2). Moreover, 
      omission of serum during differentiation process (P3) caused typical improvement 
      in functions assigned to hepatocytes in differentiated MenSCs. While 
      up-regulation level of ALB and CYP7A1 was higher in differentiated MenSCs 
      compared to driven BMSCs, expression level of CK-18, detected level of produced 
      ALB and glycogen accumulation were lower or not significantly different. 
      Therefore, based on the overall comparable hepatic differentiation ability of 
      MenSCs with BMSCs, and also accessibility, refreshing nature and lack of ethical 
      issues of MenSCs, these cells could be suggested as an apt and safe alternative 
      to BMSCs for future stem cell therapy of chronic liver diseases.
FAU - Khanjani, Sayeh
AU  - Khanjani S
AD  - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, 
      Tehran, Iran.
FAU - Khanmohammadi, Manijeh
AU  - Khanmohammadi M
AD  - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, 
      Tehran, Iran.
FAU - Zarnani, Amir-Hassan
AU  - Zarnani AH
AD  - Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, 
      Iran ; Immunology Research Center, Iran University of Medical Sciences, Tehran, 
      Iran.
FAU - Akhondi, Mohammad-Mehdi
AU  - Akhondi MM
AD  - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, 
      Tehran, Iran.
FAU - Ahani, Ali
AU  - Ahani A
AD  - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, 
      Tehran, Iran.
FAU - Ghaempanah, Zahra
AU  - Ghaempanah Z
AD  - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, 
      Tehran, Iran.
FAU - Naderi, Mohammad Mehdi
AU  - Naderi MM
AD  - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, 
      Tehran, Iran.
FAU - Eghtesad, Saman
AU  - Eghtesad S
AD  - Department of Biochemistry and Molecular Biology, University of Maryland School 
      of Medicine, Baltimore, Maryland, United States of America.
FAU - Kazemnejad, Somaieh
AU  - Kazemnejad S
AD  - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, 
      Tehran, Iran.
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Multicenter Study
DEP - 20140205
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, Differentiation)
SB  - IM
MH  - Adult
MH  - Antigens, Differentiation/*metabolism
MH  - Bone Marrow/*metabolism
MH  - *Cell Differentiation
MH  - *Hepatocytes/cytology/metabolism
MH  - Humans
MH  - *Menstrual Cycle
MH  - *Stem Cells/cytology/metabolism
PMC - PMC3914790
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2014/02/08 06:00
MHDA- 2014/11/09 06:00
PMCR- 2014/02/05
CRDT- 2014/02/08 06:00
PHST- 2013/06/06 00:00 [received]
PHST- 2013/12/05 00:00 [accepted]
PHST- 2014/02/08 06:00 [entrez]
PHST- 2014/02/08 06:00 [pubmed]
PHST- 2014/11/09 06:00 [medline]
PHST- 2014/02/05 00:00 [pmc-release]
AID - PONE-D-13-23887 [pii]
AID - 10.1371/journal.pone.0086075 [doi]
PST - epublish
SO  - PLoS One. 2014 Feb 5;9(2):e86075. doi: 10.1371/journal.pone.0086075. eCollection 
      2014.