PMID- 24505295 OWN - NLM STAT- MEDLINE DCOM- 20141108 LR - 20211021 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 9 IP - 2 DP - 2014 TI - Genome-wide analysis of antiviral signature genes in porcine macrophages at different activation statuses. PG - e87613 LID - 10.1371/journal.pone.0087613 [doi] LID - e87613 AB - Macrophages (Mcapital EF, Cyrillics) can be polarized to various activation statuses, including classical (M1), alternative (M2), and antiviral states. To study the antiviral activation status of porcine Mcapital EF, Cyrillics during porcine reproductive and respiratory syndrome virus (PRRSV) infection, we used RNA Sequencing (RNA-Seq) for transcriptomic analysis of differentially expressed genes (DEGs). Sequencing assessment and quality evaluation showed that our RNA-Seq data met the criteria for genome-wide transcriptomic analysis. Comparisons of any two activation statuses revealed more than 20,000 DEGs that were normalized to filter out 153-5,303 significant DEGs [false discovery rate (FDR) /=2] in each comparison. The highest 5,303 significant DEGs were found between lipopolysaccharide- (LPS) and interferon (IFN)gamma-stimulated M1 cells, whereas only 153 significant DEGs were detected between interleukin (IL)-10-polarized M2 cells and control mock-activated cells. To identify signature genes for antiviral regulation pertaining to each activation status, we identified a set of DEGs that showed significant up-regulation in only one activation state. In addition, pathway analyses defined the top 20-50 significantly regulated pathways at each activation status, and we further analyzed DEGs pertinent to pathways mediated by AMP kinase (AMPK) and epigenetic mechanisms. For the first time in porcine macrophages, our transcriptomic analyses not only compared family-wide differential expression of most known immune genes at different activation statuses, but also revealed transcription evidence of multiple gene families. These findings show that using RNA-Seq transcriptomic analyses in virus-infected and status-synchronized macrophages effectively profiled signature genes and gene response pathways for antiviral regulation, which may provide a framework for optimizing antiviral immunity and immune homeostasis. FAU - Sang, Yongming AU - Sang Y AD - Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America. FAU - Brichalli, Wyatt AU - Brichalli W AD - Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America. FAU - Rowland, Raymond R R AU - Rowland RR AD - Department of Diagnostic Medicine and Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America. FAU - Blecha, Frank AU - Blecha F AD - Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, United States of America. LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20140205 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Animals MH - Gene Expression Regulation/*immunology MH - Genome-Wide Association Study MH - *Macrophage Activation MH - Macrophages/*immunology/metabolism MH - Porcine Reproductive and Respiratory Syndrome/*immunology/metabolism/pathology MH - Porcine respiratory and reproductive syndrome virus/*immunology/*metabolism MH - Swine PMC - PMC3914820 COIS- Competing Interests: The authors have declared that no competing interests exist. EDAT- 2014/02/08 06:00 MHDA- 2014/11/09 06:00 PMCR- 2014/02/05 CRDT- 2014/02/08 06:00 PHST- 2013/11/04 00:00 [received] PHST- 2013/12/20 00:00 [accepted] PHST- 2014/02/08 06:00 [entrez] PHST- 2014/02/08 06:00 [pubmed] PHST- 2014/11/09 06:00 [medline] PHST- 2014/02/05 00:00 [pmc-release] AID - PONE-D-13-45022 [pii] AID - 10.1371/journal.pone.0087613 [doi] PST - epublish SO - PLoS One. 2014 Feb 5;9(2):e87613. doi: 10.1371/journal.pone.0087613. eCollection 2014.