PMID- 2450577
OWN - NLM
STAT- MEDLINE
DCOM- 19880505
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 27
IP  - 2
DP  - 1988 Jan 26
TI  - Antibodies to peptide determinants in transforming growth factor beta and their 
      applications.
PG  - 739-46
AB  - Polyclonal antibodies have been raised to a series of synthetic peptides which 
      correspond to essentially all regions of the transforming growth factor beta 1 
      (TGF-beta 1) molecule. All antisera were evaluated for their abilities to react 
      with TGF-beta 1 and TGF-beta 2 in either the native or reduced form in 
      enzyme-linked immunosorbent assays, Western blots, and immunoprecipitation 
      assays. While all antisera demonstrated some ability to recognize TGF-beta 1 in 
      these systems, there was limited cross-reactivity with TGF-beta 2, suggesting 
      that substantial sequence or conformational differences exist between the two 
      growth factors. On Western blots 5-10 ng of purified human platelet TGF-beta 1 
      could be detected when probed with affinity-purified peptide antisera generated 
      against peptides corresponding to residues 48-77, 50-75, and 78-109 of the 112 
      amino acid TGF-beta 1 monomer. Antisera raised against peptides 50-75 and 78-109 
      were most effective in immunoprecipitating reduced and native 125I-TGF-beta 1, 
      respectively. The antisera also were tested for their effectiveness in blocking 
      the binding of 125I-TGF-beta 1 to its receptor. Anti-peptide 78-109 and 
      anti-peptide 50-75 blocked 80% and 40% of the binding, respectively, while 
      antibodies against amino-terminal peptides were without effect. These data 
      suggest that the carboxyl-terminal region of TGF-beta 1 may play a significant 
      role in the binding of the native ligand to its receptor.
FAU - Flanders, K C
AU  - Flanders KC
AD  - Laboratory of Chemoprevention, National Cancer Institute, Bethesda, Maryland 
      20892.
FAU - Roberts, A B
AU  - Roberts AB
FAU - Ling, N
AU  - Ling N
FAU - Fleurdelys, B E
AU  - Fleurdelys BE
FAU - Sporn, M B
AU  - Sporn MB
LA  - eng
GR  - 5F32AM07423/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Antibodies)
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Epitopes)
RN  - 0 (Growth Substances)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Peptides)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 76057-06-2 (Transforming Growth Factors)
SB  - IM
MH  - Animals
MH  - *Antibodies
MH  - Antigen-Antibody Complex
MH  - Cell Line
MH  - Cross Reactions
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes/*analysis
MH  - Growth Substances/*immunology
MH  - Immunoglobulin G
MH  - Kinetics
MH  - Peptides/analysis/*immunology/metabolism
MH  - Radioimmunoassay
MH  - Receptors, Cell Surface/metabolism
MH  - Receptors, Transforming Growth Factor beta
MH  - Transforming Growth Factors
EDAT- 1988/01/26 00:00
MHDA- 1988/01/26 00:01
CRDT- 1988/01/26 00:00
PHST- 1988/01/26 00:00 [pubmed]
PHST- 1988/01/26 00:01 [medline]
PHST- 1988/01/26 00:00 [entrez]
AID - 10.1021/bi00402a037 [doi]
PST - ppublish
SO  - Biochemistry. 1988 Jan 26;27(2):739-46. doi: 10.1021/bi00402a037.