PMID- 24507038
OWN - NLM
STAT- MEDLINE
DCOM- 20150115
LR  - 20181202
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 46
IP  - 1
DP  - 2014 Jan-Feb
TI  - Stable expression of hypoxia-inducible factor-1alpha in human renal proximal tubular 
      epithelial cells promotes epithelial to mesenchymal transition.
PG  - 130-4
LID - S0041-1345(13)01152-4 [pii]
LID - 10.1016/j.transproceed.2013.06.024 [doi]
AB  - BACKGROUND: Late kidney allograft dysfunction is becoming a significant problem 
      and tubular atrophy and interstitial fibrosis are main causes. It was reported 
      that hypoxia could induce epithelial-mesenchymal transition (EMT) in renal 
      tubular epithelial cells (TECs), and hypoxia-inducible factor-1 (HIF-1) is one of 
      the important regulators of cellular adaptive to hypoxia. METHODS: In this study, 
      we used an HIF-1alphaDeltaODD-expressing adenovirus, which could stably and functionally 
      express HIF-1alpha under normoxia conditions, and used a hypoxia/reoxygenation cell 
      model to simulate ischemia/reperfusion (I/R) injury in vitro, to investigate the 
      effect of HIF-1alpha on EMT-related gene expressions. RESULTS: Our results 
      demonstrated that HIF-1alpha could significantly upregulate alpha-smooth muscle actin 
      expression, and reduced the E-cadherin expression in HK-2 cells during I/R 
      injury. Moreover, miR-21 expression had a positive correlation with HIF-1alpha in 
      this process. CONCLUSION: These results suggest that HIF-1alpha may promote the EMT 
      development through regulating fibrotic gene expression during I/R injury in 
      human renal TECs, and miR-21 could be among the important regulatory pathways in 
      the process.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Luo, L
AU  - Luo L
AD  - Department of Research and Education, Guizhou Province People's Hospital, 
      Guiyang, China.
FAU - Luo, G
AU  - Luo G
AD  - Department of Urology Surgery, Guizhou Province People's Hospital, Guiyang, 
      China.
FAU - Fang, Q
AU  - Fang Q
AD  - Department of Nursing, Guizhou Province People's Hospital, Guiyang, China.
FAU - Sun, Z
AU  - Sun Z
AD  - Department of Urology Surgery, Guizhou Province People's Hospital, Guiyang, 
      China. Electronic address: szl7710@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Actins)
RN  - 0 (Antigens, CD)
RN  - 0 (CDH1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Actins/metabolism
MH  - Adenoviridae/genetics/metabolism
MH  - Antigens, CD
MH  - Cadherins/metabolism
MH  - Cell Line
MH  - *Epithelial-Mesenchymal Transition
MH  - *Gene Expression Regulation
MH  - Graft Survival
MH  - Humans
MH  - Hypoxia/pathology
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Kidney/pathology
MH  - Kidney Transplantation
MH  - Kidney Tubules/*cytology
MH  - MicroRNAs/metabolism
MH  - Muscle, Smooth/metabolism
MH  - Oxygen/metabolism
MH  - RNA Interference
MH  - Reperfusion Injury
MH  - Up-Regulation
EDAT- 2014/02/11 06:00
MHDA- 2015/01/16 06:00
CRDT- 2014/02/11 06:00
PHST- 2012/10/23 00:00 [received]
PHST- 2013/06/28 00:00 [accepted]
PHST- 2014/02/11 06:00 [entrez]
PHST- 2014/02/11 06:00 [pubmed]
PHST- 2015/01/16 06:00 [medline]
AID - S0041-1345(13)01152-4 [pii]
AID - 10.1016/j.transproceed.2013.06.024 [doi]
PST - ppublish
SO  - Transplant Proc. 2014 Jan-Feb;46(1):130-4. doi: 
      10.1016/j.transproceed.2013.06.024.