PMID- 24507659
OWN - NLM
STAT- MEDLINE
DCOM- 20140922
LR  - 20211021
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 10
DP  - 2014 Feb 8
TI  - Anti-idiotypic antibodies reduce efficacy of the attenuated vaccine against 
      highly pathogenic PRRSV challenge.
PG  - 39
LID - 10.1186/1746-6148-10-39 [doi]
AB  - BACKGROUND: The inability of current vaccines to provide effective protection 
      against porcine reproductive and respiratory syndrome virus (PRRSV) infection is 
      not fully understood. One of the reasons might be the presence of anti-idiotypic 
      antibodies (Ab2s) to the envelope glycoprotein GP5 induced by PRRSV infection 
      since our previous studies demonstrated the presence of auto-Ab2s (aAb2s) in pigs 
      infected with PRRSV. To test this hypothesis, PRRSV negative piglets were 
      injected with a monoclonal Ab2 (Mab2-5G2) and aAb2s that are specific for 
      anti-GP5 antibody, vaccinated with the attenuated PRRSV vaccine CH-1R and then 
      challenged with the highly pathogenic PRRSV HuN4 strain. The animals were 
      evaluated for clinical signs, pathological changes of the thymus and lungs, 
      viremia, levels of serum antibodies and cytokines. RESULTS: The piglets injected 
      with Mab2-5G2 or aAb2, and who received the attenuated PRRSV vaccine CH-1R before 
      challenge, produced high levels of anti-N antibodies, IL-2 and IL-4, but low 
      levels of neutralizing antibodies. After PRRSV HuN4 challenge, the animals showed 
      obvious clinical signs, including lung lesions, severe thymus atrophy and 
      decreased production of IL-4 and higher level of viremia. CONCLUSION: When 
      anti-GP5 Ab2s are present, the use of attenuated PRRSV vaccine CH-1R against 
      HP-PRRSV infection is not recommended. It can result in poor health status with 
      pneumonia and thymus atrophy.
FAU - Yu, Ying
AU  - Yu Y
FAU - Cai, Xuehui
AU  - Cai X
FAU - Wang, Gang
AU  - Wang G
FAU - Kong, Ning
AU  - Kong N
FAU - Liu, Yonggang
AU  - Liu Y
FAU - Xiao, Yihong
AU  - Xiao Y
FAU - Zhang, Chong
AU  - Zhang C
FAU - Mu, Yang
AU  - Mu Y
FAU - Xiao, Shuqi
AU  - Xiao S
FAU - Zhao, Qin
AU  - Zhao Q
FAU - Wang, Chengbao
AU  - Wang C
FAU - Zhang, Gaiping
AU  - Zhang G
FAU - Hiscox, Julian A
AU  - Hiscox JA
FAU - Zhou, En-Min
AU  - Zhou EM
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling, Shaanxi 712100, China. zhouem@nwsuaf.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20140208
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Vaccines, Attenuated/immunology
MH  - Viral Vaccines/*immunology
PMC - PMC3921987
EDAT- 2014/02/11 06:00
MHDA- 2014/09/23 06:00
PMCR- 2014/02/08
CRDT- 2014/02/11 06:00
PHST- 2013/10/14 00:00 [received]
PHST- 2014/01/29 00:00 [accepted]
PHST- 2014/02/11 06:00 [entrez]
PHST- 2014/02/11 06:00 [pubmed]
PHST- 2014/09/23 06:00 [medline]
PHST- 2014/02/08 00:00 [pmc-release]
AID - 1746-6148-10-39 [pii]
AID - 10.1186/1746-6148-10-39 [doi]
PST - epublish
SO  - BMC Vet Res. 2014 Feb 8;10:39. doi: 10.1186/1746-6148-10-39.