PMID- 2450797
OWN - NLM
STAT- MEDLINE
DCOM- 19880425
LR  - 20190721
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 126
IP  - 2
DP  - 1988 Apr
TI  - Neuronal precursor cells in chick dorsal root ganglia: differentiation and 
      survival in vitro.
PG  - 420-32
AB  - Neuronal precursor cells present in dorsal root ganglia (DRG) during early 
      development have been previously shown to differentiate in vitro to neurons, as 
      characterized by morphology, cell surface antigens, and electrophysiological 
      properties (H. Rohrer, S. Henke-Fahle, T. El-Sharkawy, H. D. Lux, and H. Thoenen, 
      1985, Embo J. 4, 1709-1714). In the present study the conditions necessary for 
      the initial differentiation and long-term survival of these cells were 
      established, and the neurotransmitter phenotype of the newly differentiated 
      neurons was analyzed. Neuronal precursor cells isolated from chick DRG at 
      Embryonic Day 6 (E6) were found to require the presence of a polyornithine 
      substrate coated with either laminin or fibronectin for initial neurite 
      production and long-term survival. Neurons were unable to develop on 
      polyornithine alone or on polyornithine coated with BSA. The survival and neurite 
      outgrowth from neuronal precursor cells was not affected by the presence of nerve 
      growth factor (NGF) during the first 9 hr in culture. NGF also had no effect on 
      the proportion of cells expressing the neuron-specific Q211 antigen. However, 
      after this initial differentiation period the neurons did require the presence of 
      a survival factor. The neurons could be maintained for at least 6 days in culture 
      both in the presence of NGF and in the presence of brain-derived neurotrophic 
      factor (BDNF). At saturating concentrations of both survival factors no additive 
      effects could be observed, indicating a complete overlap of NGF- and 
      BDNF-responsiveness. Although the same proportion of cells survived with either 
      NGF or BDNF during the first 3 days in culture, survival decreased in the 
      presence of BDNF but not in the presence of NGF during the following 3 days in 
      culture. The loss of BDNF responsiveness in vitro was also observed in vivo. 
      After 6 days in culture about 70% of the neurons expressed substance P 
      immunoreactivity, and approximately the same proportion was positive for 
      myelin-associated glycoprotein immunoreactivity. The neurons did not express 
      properties of adrenergic neurons such as tyrosine hydroxylase immunoreactivity or 
      norepinephrine uptake. These findings indicate that the neuronal precursor cells 
      from E6 DRG acquire the same characteristics in vitro as in their normal in vivo 
      environment.
FAU - Ernsberger, U
AU  - Ernsberger U
AD  - Max-Planck-Institute for Psychiatry, Department of Neurochemistry, Martinsried, 
      Federal Republic of Germany.
FAU - Rohrer, H
AU  - Rohrer H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Epitopes)
RN  - 0 (Globosides)
RN  - 0 (Glycolipids)
RN  - 0 (Lewis X Antigen)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 33507-63-0 (Substance P)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Epitopes/analysis
MH  - Ganglia, Spinal/*embryology
MH  - Globosides/analysis
MH  - Glycolipids/analysis/genetics
MH  - Immunohistochemistry
MH  - Lewis X Antigen
MH  - Nerve Growth Factors/pharmacology
MH  - Nerve Tissue Proteins/pharmacology
MH  - Neurons/*cytology/drug effects
MH  - Neurons, Afferent/cytology/drug effects
MH  - Norepinephrine/metabolism
MH  - Substance P/analysis
EDAT- 1988/04/01 00:00
MHDA- 1988/04/01 00:01
CRDT- 1988/04/01 00:00
PHST- 1988/04/01 00:00 [pubmed]
PHST- 1988/04/01 00:01 [medline]
PHST- 1988/04/01 00:00 [entrez]
AID - 0012-1606(88)90151-0 [pii]
AID - 10.1016/0012-1606(88)90151-0 [doi]
PST - ppublish
SO  - Dev Biol. 1988 Apr;126(2):420-32. doi: 10.1016/0012-1606(88)90151-0.