PMID- 24508265
OWN - NLM
STAT- MEDLINE
DCOM- 20140502
LR  - 20140303
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 445
IP  - 1
DP  - 2014 Feb 28
TI  - Green tea catechins potentiate the neuritogenic action of brain-derived 
      neurotrophic factor: role of 67-kDa laminin receptor and hydrogen peroxide.
PG  - 218-24
LID - S0006-291X(14)00209-5 [pii]
LID - 10.1016/j.bbrc.2014.01.166 [doi]
AB  - Delivery of optimal amounts of brain-derived neurotrophic factor (BDNF) to 
      regions of the brain affected by neurodegenerative diseases is a daunting task. 
      Using natural products with neuroprotective properties, such as green tea 
      polyphenols, would be a highly useful complementary approach for inexpensive 
      long-term treatment of these diseases. In this study, we used PC12(TrkB) cells 
      which ectopically express TrkB, a high affinity receptor for BDNF. They 
      differentiate and induce neurite outgrowth in response to BDNF. Using this model, 
      we show for the first time that treatment with extremely low concentrations (<0.1 
      mug/ml) of unfractionated green tea polyphenols (GTPP) and low concentrations 
      (<0.5 muM) of their active ingredient, epigallocatechin-3-gallate (EGCG), 
      potentiated the neuritogenic ability of a low concentration (2 ng/ml) of BDNF. A 
      synergistic interaction was observed between GTPP constituents, where 
      epigallocatechin and epicatechin, both individually lacking this activity, 
      promoted the action of EGCG. GTPP-induced potentiation of BDNF action required 
      the cell-surface associated 67 kDa laminin receptor (67LR) to which EGCG binds 
      with high affinity. A cell-permeable catalase abolished GTPP/EGCG-induced 
      potentiation of BDNF action, suggesting the possible involvement of H2O2 in the 
      potentiation. Consistently, exogenous sublethal concentrations of H2O2, added as 
      a bolus dose (5 muM) or more effectively through a steady-state generation (1 muM), 
      potentiated BDNF action. Collectively, these results suggest that EGCG, dependent 
      on 67 LR and H2O2, potentiates the neuritogenic action of BDNF. Intriguingly, 
      this effect requires only submicromolar concentrations of EGCG. This is 
      significant as extremely low concentrations of polyphenols are believed to reach 
      the brain after drinking green tea.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Gundimeda, Usha
AU  - Gundimeda U
AD  - Department of Cell and Neurobiology, Keck School of Medicine, University of 
      Southern California, Los Angeles, CA 90089, USA.
FAU - McNeill, Thomas H
AU  - McNeill TH
AD  - Department of Cell and Neurobiology, Keck School of Medicine, University of 
      Southern California, Los Angeles, CA 90089, USA.
FAU - Fan, Tiffany K
AU  - Fan TK
AD  - Department of Cell and Neurobiology, Keck School of Medicine, University of 
      Southern California, Los Angeles, CA 90089, USA.
FAU - Deng, Ronald
AU  - Deng R
AD  - Department of Cell and Neurobiology, Keck School of Medicine, University of 
      Southern California, Los Angeles, CA 90089, USA.
FAU - Rayudu, David
AU  - Rayudu D
AD  - Department of Cell and Neurobiology, Keck School of Medicine, University of 
      Southern California, Los Angeles, CA 90089, USA.
FAU - Chen, Zachary
AU  - Chen Z
AD  - Department of Cell and Neurobiology, Keck School of Medicine, University of 
      Southern California, Los Angeles, CA 90089, USA.
FAU - Cadenas, Enrique
AU  - Cadenas E
AD  - Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, 
      University of Southern California, Los Angeles, CA 90089, USA.
FAU - Gopalakrishna, Rayudu
AU  - Gopalakrishna R
AD  - Department of Cell and Neurobiology, Keck School of Medicine, University of 
      Southern California, Los Angeles, CA 90089, USA. Electronic address: 
      rgopalak@usc.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140204
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antioxidants)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Oxidants)
RN  - 0 (Polyphenols)
RN  - 0 (Receptors, Laminin)
RN  - 0 (Tea)
RN  - 8R1V1STN48 (Catechin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - BQM438CTEL (epigallocatechin gallate)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Catechin/analogs & derivatives/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Hydrogen Peroxide/metabolism/pharmacology
MH  - Molecular Weight
MH  - Neurites/*drug effects/physiology
MH  - Oxidants/metabolism/pharmacology
MH  - PC12 Cells
MH  - Polyphenols/pharmacology
MH  - Rats
MH  - Receptor, trkB/genetics/metabolism
MH  - Receptors, Laminin/chemistry/metabolism/physiology
MH  - Tea/*chemistry
OTO - NOTNLM
OT  - 67-kDa laminin receptor
OT  - Brain-derived neurotrophic factor
OT  - Epigallocatechin-3-gallate
OT  - Green tea polyphenol
OT  - Hydrogen peroxide
OT  - Neuritogenesis
EDAT- 2014/02/11 06:00
MHDA- 2014/05/03 06:00
CRDT- 2014/02/11 06:00
PHST- 2014/01/20 00:00 [received]
PHST- 2014/01/28 00:00 [accepted]
PHST- 2014/02/11 06:00 [entrez]
PHST- 2014/02/11 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
AID - S0006-291X(14)00209-5 [pii]
AID - 10.1016/j.bbrc.2014.01.166 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2014 Feb 28;445(1):218-24. doi: 
      10.1016/j.bbrc.2014.01.166. Epub 2014 Feb 4.