PMID- 24508785
OWN - NLM
STAT- MEDLINE
DCOM- 20140506
LR  - 20220330
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Linking)
VI  - 55
IP  - 3
DP  - 2014 Mar 4
TI  - Activation of the prostanoid FP receptor inhibits adipogenesis leading to 
      deepening of the upper eyelid sulcus in prostaglandin-associated periorbitopathy.
PG  - 1269-76
LID - 10.1167/iovs.13-12589 [doi]
AB  - PURPOSE: To investigate the effects of prostaglandin (PG) analogues on 
      adipogenesis so as to clarify the mechanism of a side effect of topical PG 
      analogues: deepening of the upper eyelid sulcus (DUES), which has been reported 
      in this decade. METHODS: The 3T3-L1 preadipocytes were treated to promote 
      differentiation into mature adipocytes. During the early and late stages of 
      differentiation (days 0, 2, and 7), 1 to 1000 nM latanoprost acid (LAT-A), 
      travoprost acid (TRA-A), tafluprost acid (TAF-A), bimatoprost (BIM), bimatoprost 
      acid (BIM-A), unoprostone (UNO), or prostaglandin F2a (PGF2alpha) was applied to 
      cells. Oil red O staining was used to detect intracellular lipids on day 10. 
      Stained areas measured on a photograph were compared with those in control 
      cultures. All experiments were performed in a masked manner. Next, similar 
      experiments were performed using primary cultured mouse adipocytes from FP 
      receptor knockout and wild-type mice. RESULTS: When PGs were added on day 0 or 2, 
      LAT-A, TAF-A, BIM-A, and PGF2alpha significantly inhibited adipogenesis (P < 0.01 on 
      day 0, P < 0.05 on day 2) at concentrations of 10 nM and 100 nM, and TRA-A 
      inhibited adipogenesis only at 100 nM. Bimatoprost and UNO did not affect 
      adipogenesis at any concentration. When PGs were added on day 7, 100 nM LAT-A, 
      BIM-A, or PGF2alpha significantly suppressed adipogenesis (P < 0.05). In mouse 
      primary adipocyte cultures, LAT-A, TAF-A, BIM-A, TRA-A, and PGF2alpha significantly 
      suppressed adipogenesis in wild-type adipocytes (P < 0.05), but adipogenesis was 
      not suppressed by any of the PG compounds in FP knockout mouse adipocytes. 
      CONCLUSIONS: Prostaglandin analogues have the potential to inhibit adipogenesis 
      through FP receptor stimulation. Although these findings should be further 
      analyzed in model systems more closely related to orbital fat, PG analogues may 
      directly lead to reduced orbital fat by inhibiting adipogenesis.
FAU - Taketani, Yukako
AU  - Taketani Y
AD  - Department of Ophthalmology, University of Tokyo, Tokyo, Japan.
FAU - Yamagishi, Reiko
AU  - Yamagishi R
FAU - Fujishiro, Takashi
AU  - Fujishiro T
FAU - Igarashi, Masaki
AU  - Igarashi M
FAU - Sakata, Rei
AU  - Sakata R
FAU - Aihara, Makoto
AU  - Aihara M
LA  - eng
PT  - Journal Article
DEP - 20140304
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Prostaglandins, Synthetic)
RN  - 0 (Receptors, Prostaglandin)
RN  - 0 (prostaglandin F2alpha receptor)
SB  - IM
MH  - Adipocytes/drug effects/pathology
MH  - Adipogenesis/*drug effects
MH  - Animals
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Eyelids/*drug effects/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Orbit/*pathology
MH  - Orbital Diseases/chemically induced/*metabolism/pathology
MH  - Prostaglandins, Synthetic
MH  - Receptors, Prostaglandin/*metabolism
OTO - NOTNLM
OT  - FP receptor
OT  - deepening of the upper eyelid sulcus
OT  - lipids
OT  - prostaglandins
EDAT- 2014/02/11 06:00
MHDA- 2014/05/07 06:00
CRDT- 2014/02/11 06:00
PHST- 2014/02/11 06:00 [entrez]
PHST- 2014/02/11 06:00 [pubmed]
PHST- 2014/05/07 06:00 [medline]
AID - iovs.13-12589 [pii]
AID - 10.1167/iovs.13-12589 [doi]
PST - epublish
SO  - Invest Ophthalmol Vis Sci. 2014 Mar 4;55(3):1269-76. doi: 10.1167/iovs.13-12589.