PMID- 24509065
OWN - NLM
STAT- MEDLINE
DCOM- 20141204
LR  - 20140407
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 45
IP  - 1
DP  - 2014 Jul
TI  - Fish granulocytes express a constitutively active androgen receptor variant.
PG  - 115-22
LID - S0145-305X(14)00031-7 [pii]
LID - 10.1016/j.dci.2014.01.024 [doi]
AB  - In humans, alternative splicing of androgen receptor (AR) is usually involved in 
      some diseases. However, our knowledge about the presence of AR variants in other 
      species and its importance for immunity is scant. Here, we report the 
      identification of a constitutively active AR variant lacking the ligand-binding 
      domain (LBD), ARDeltaLBD, in the fish gilthead seabream. ARDeltaLBD is expressed in the 
      testis and the head-kidney (HK), and its expression varies with the reproductive 
      stage and is correlated with plasma testosterone (T). In addition, ARDeltaLBD is 
      expressed in acidophilic granulocytes (AGs), which are the functional equivalent 
      of mammalian neutrophils, but not in macrophages, and its expression is modulated 
      by both T and immune stimuli. Notably, AR and ARDeltaLBD were able to interact, being 
      the activity of AR dominant at all concentrations tested of the ligand. These 
      results reveal a new mechanism for the regulation of neutrophil biology in 
      vertebrates and explain the conflicting results that suggest that androgens are 
      less important than AR in human and mouse neutrophil homeostasis.
CI  - Copyright (c) 2014 Elsevier Ltd. All rights reserved.
FAU - Sanchez-Hernandez, Miriam
AU  - Sanchez-Hernandez M
AD  - Centro Oceanografico de Murcia, Instituto Espanol de Oceanografia (IEO), 
      Carretera de la Azohia s/n, Puerto de Mazarron, 30860 Murcia, Spain.
FAU - Arizcun, Marta
AU  - Arizcun M
AD  - Centro Oceanografico de Murcia, Instituto Espanol de Oceanografia (IEO), 
      Carretera de la Azohia s/n, Puerto de Mazarron, 30860 Murcia, Spain.
FAU - Garcia-Alcazar, Alicia
AU  - Garcia-Alcazar A
AD  - Centro Oceanografico de Murcia, Instituto Espanol de Oceanografia (IEO), 
      Carretera de la Azohia s/n, Puerto de Mazarron, 30860 Murcia, Spain.
FAU - Sarropoulou, Elena
AU  - Sarropoulou E
AD  - Institute of Marine Biology and Genetics, Hellenic Center for Marine Research, 
      Crete, Greece.
FAU - Mulero, Victoriano
AU  - Mulero V
AD  - Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of 
      International Excellence "Campus Mare Nostrum", University of Murcia, 30100 
      Murcia, Spain. Electronic address: vmulero@um.es.
FAU - Garcia-Ayala, Alfonsa
AU  - Garcia-Ayala A
AD  - Department of Cell Biology and Histology, Faculty of Biology, Regional Campus of 
      International Excellence "Campus Mare Nostrum", University of Murcia, 30100 
      Murcia, Spain. Electronic address: agayala@um.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140206
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Fish Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Androgen)
RN  - 3XMK78S47O (Testosterone)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Fish Proteins/genetics/*metabolism
MH  - Gene Expression
MH  - Granulocytes/*metabolism
MH  - HEK293 Cells
MH  - Head Kidney/cytology
MH  - Humans
MH  - Male
MH  - Protein Isoforms/genetics/metabolism
MH  - Receptors, Androgen/genetics/*metabolism
MH  - Sea Bream/*anatomy & histology/metabolism
MH  - Testis/metabolism
MH  - Testosterone/blood
MH  - Transcriptional Activation
OTO - NOTNLM
OT  - Alternative splicing
OT  - Androgen receptor
OT  - Gilthead seabream
OT  - Leukocytes
OT  - Testis
OT  - Testosterone
EDAT- 2014/02/11 06:00
MHDA- 2014/12/15 06:00
CRDT- 2014/02/11 06:00
PHST- 2013/11/25 00:00 [received]
PHST- 2014/01/27 00:00 [revised]
PHST- 2014/01/30 00:00 [accepted]
PHST- 2014/02/11 06:00 [entrez]
PHST- 2014/02/11 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - S0145-305X(14)00031-7 [pii]
AID - 10.1016/j.dci.2014.01.024 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2014 Jul;45(1):115-22. doi: 10.1016/j.dci.2014.01.024. Epub 
      2014 Feb 6.