PMID- 24510328
OWN - NLM
STAT- MEDLINE
DCOM- 20140311
LR  - 20141212
IS  - 1833-3516 (Print)
IS  - 1833-3516 (Linking)
VI  - 43
IP  - 4
DP  - 2013 Dec
TI  - Hyperbaric oxygen therapy treatment for the recovery of muscle injury induced in 
      rats.
PG  - 222-5
AB  - INTRODUCTION: We evaluated the effect of hyperbaric oxygen treatment (HBOT) in 
      the recovery of muscle injury in rats. MATERIALS AND METHODS: Twelve female 
      Wistar rats, weighing 200-250 g, were submitted to contusion of the right 
      gastrocnemius muscle. Animals were then randomly assigned to an untreated control 
      group or an HBOT-treated group. The HBOT group was given three, 60-minute 
      sessions of HBOT at 253 kPa pressure at 24, 48 and 72 hours post injury. After 
      the last session all animals were sacrificed and both gastrocnemius muscles 
      removed, the left muscle as an internal control. Blood samples were taken for 
      creatine phosphokinase (CPK). Using a standard traction technique, the muscles 
      were analysed for their biomechanical properties: hardness, maximum elongation 
      and maximum weight. RESULTS: Significant differences were found between uninjured 
      and injured muscles and between untreated and HBOT groups in maximum weight and 
      hardness: maximum weight in the non-treated group 18.27 +/- 2.99 N versus 26.18 +/- 
      2.84 N in the HBOT group (P = 0.007); hardness in the non-treated group 2.24 +/- 
      0.38 103 N m(-)(1) versus 3.19 +/- 0.32 10(3) N m(-)(1) in the HBOT group (P = 0.001). The 
      difference in maximum elongation was not significant (P = 0.793). CPK was 
      significantly different between the two groups (non-treated 6,445 +/- 387 i.u. L(-)(1); 
      HBOT 4,551 +/- 80 i.u. L(-)(1); P = 0.009). CONCLUSIONS: HBOT seems to play a positive 
      role in the recovery of induced muscle injury in rats. However relevant, these 
      results cannot be extrapolated to humans, for whom further clinical studies are 
      warranted.
FAU - Cervaens Costa Maia, Mariana
AU  - Cervaens Costa Maia M
AD  - Faculty of Health Sciences, Fernando Pessoa University, Porto, Portugal.
FAU - Camacho, Oscar Ferraz
AU  - Camacho OF
AD  - Hyperbaric Medicine Unit, Pedro Hispano Hospital, Matosinhos, Portugal.
FAU - Pinto Marques, Agostinho Franklim
AU  - Pinto Marques AF
AD  - Faculty of Pharmacy, University of Porto, Porto, Portugal.
FAU - Barata de Silva Coelho, Pedro Miguel
AU  - Barata de Silva Coelho PM
AD  - Faculty of Health Sciences, Fernando Pessoa University, Faculdade de Ciencias da 
      Saude da Universidade Fernando Pessoa Rua Carlos da Maia 296, 4200 Porto 
      Portugal, Phone: +35-(0)12-2507-1300, Fax: +35-(0)2250-7109, E-mail: 
      pbarata@ufp.edu.pt.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Diving Hyperb Med
JT  - Diving and hyperbaric medicine
JID - 101282742
RN  - EC 2.7.3.2 (Creatine Kinase)
SB  - IM
MH  - Animals
MH  - Biomechanical Phenomena/physiology
MH  - Contusions/blood/enzymology/physiopathology/*therapy
MH  - Creatine Kinase/blood
MH  - Female
MH  - Hyperbaric Oxygenation/*methods
MH  - Muscle, Skeletal/*injuries/pathology/physiopathology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
OTO - NOTNLM
OT  - Hyperbaric oxygen
OT  - hyperbaric oxygen therapy
OT  - injuries
OT  - musculo-skeletal
OT  - research
EDAT- 2014/02/11 06:00
MHDA- 2014/03/13 06:00
CRDT- 2014/02/11 06:00
PHST- 2012/12/18 00:00 [received]
PHST- 2013/10/23 00:00 [accepted]
PHST- 2014/02/11 06:00 [entrez]
PHST- 2014/02/11 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
PST - ppublish
SO  - Diving Hyperb Med. 2013 Dec;43(4):222-5.